PPS006

antibody from R&D Systems

Targeting: GAP43 B-50, GAP-43, PP46

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PPS006

Provider

R&D Systems

Product name

Phospho-GAP-43 (S41) Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Specific for the ~50 kDa GAP-43 protein phosphorylated at S41 in Western blots. In some tissues the antibody also recognizes a higher molecular weight protein, that is also recognized by the Pan GAP-43 antibody. This may be a GAP-43 aggregate or oligomer.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Simian, Xenopus, Zebrafish

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 uL

Storage

For long-term storage,

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Phospho-GAP-43 (S41) by Western blot. Western blot of rat cortex lysate showing specific immunolabeling of the ~50 kDa GAP-43 protein phosphorylated at S41 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda-PPase) for 30 minutes before being exposed to the Anti-Phospho-GAP-43 (S41). The immunolabeling is completely eliminated by treatment with lambda-PPase.