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- References [9]
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- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 21527-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#21527-1-AP, RRID:AB_10734432
- Product name
- MYLK3 antibody
- Antibody type
- Polyclonal
- Description
- MYLK3 antibody (Cat. #21527-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Epac1 increases myosin regulatory light-chain phosphorylation, energetic cost of contraction, and susceptibility to heart failure.
Proline rich-39 (PR-39) antimicrobial protein alleviated lipopolysaccharide-induced intestinal barrier dysfunction in piglets by altering intestinal flora associated bile acid metabolism and in turn regulating TGR-5/NF-κB/MLCK/MLC pathway.
Betulinic Acid Reduces Intestinal Inflammation and Enhances Intestinal Tight Junctions by Modulating the PPAR-γ/NF-κB Signaling Pathway in Intestinal Cells and Organoids.
Bisdemethoxycurcumin and Curcumin Alleviate Inflammatory Bowel Disease by Maintaining Intestinal Epithelial Integrity and Regulating Gut Microbiota in Mice.
Qula-derived Limosilactobacillus fermentum TD-3 and Lactococcus lactis MQ1-1 alleviate aging-related intestinal barrier dysfunction via microbiota-short-chain fatty acid-AMPK/MLCK-tight junction axis.
GYY4137, as a slow-releasing H(2)S donor, ameliorates sodium deoxycholate-induced chronic intestinal barrier injury and gut microbiota dysbiosis.
Capture of RNA-binding proteins across mouse tissues using HARD-AP.
GPR65 inhibits human trophoblast cell adhesion through upregulation of MYLK and downregulation of fibronectin via cAMP-ERK signaling in a low pH environment.
Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.
Ohnuki Y, Suita K, Ishikawa M, Mototani Y, Nariyama M, Ito A, Matsuo I, Hayakawa Y, Morii A, Mitsubayashi T, Saeki Y, Ishikawa Y, Okumura S
PloS one 2025;20(6):e0325986
PloS one 2025;20(6):e0325986
Proline rich-39 (PR-39) antimicrobial protein alleviated lipopolysaccharide-induced intestinal barrier dysfunction in piglets by altering intestinal flora associated bile acid metabolism and in turn regulating TGR-5/NF-κB/MLCK/MLC pathway.
Liu Z, Qin X, Zhang B, Nong K, Chen W, Yang Z, Lang W, Liu X, Li L, Wang X, Shi H, Zhang H
International journal of biological macromolecules 2025 May;307(Pt 1):141930
International journal of biological macromolecules 2025 May;307(Pt 1):141930
Betulinic Acid Reduces Intestinal Inflammation and Enhances Intestinal Tight Junctions by Modulating the PPAR-γ/NF-κB Signaling Pathway in Intestinal Cells and Organoids.
Zheng X, Cao Z, Wang M, Yuan R, Han Y, Li A, Wang X
Nutrients 2025 Jun 20;17(13)
Nutrients 2025 Jun 20;17(13)
Bisdemethoxycurcumin and Curcumin Alleviate Inflammatory Bowel Disease by Maintaining Intestinal Epithelial Integrity and Regulating Gut Microbiota in Mice.
Hsu KY, Majeed A, Ho CT, Pan MH
Journal of agricultural and food chemistry 2025 Feb 12;73(6):3494-3506
Journal of agricultural and food chemistry 2025 Feb 12;73(6):3494-3506
Qula-derived Limosilactobacillus fermentum TD-3 and Lactococcus lactis MQ1-1 alleviate aging-related intestinal barrier dysfunction via microbiota-short-chain fatty acid-AMPK/MLCK-tight junction axis.
Liu X, Guan K, Liu C, Sun Y, Ma Y, Mao K, Li Q, Wang R, Lu W
Journal of dairy science 2025 Dec;108(12):12949-12969
Journal of dairy science 2025 Dec;108(12):12949-12969
GYY4137, as a slow-releasing H(2)S donor, ameliorates sodium deoxycholate-induced chronic intestinal barrier injury and gut microbiota dysbiosis.
Pan S, Yan H, Zhu J, Ma Y, Wang P, Liu Y, Chen Z
Frontiers in pharmacology 2024;15:1476407
Frontiers in pharmacology 2024;15:1476407
Capture of RNA-binding proteins across mouse tissues using HARD-AP.
Ren Y, Liao H, Yan J, Lu H, Mao X, Wang C, Li YF, Liu Y, Chen C, Chen L, Wang X, Zhou KY, Liu HM, Liu Y, Hua YM, Yu L, Xue Z
Nature communications 2024 Sep 28;15(1):8421
Nature communications 2024 Sep 28;15(1):8421
GPR65 inhibits human trophoblast cell adhesion through upregulation of MYLK and downregulation of fibronectin via cAMP-ERK signaling in a low pH environment.
Mao J, Feng Y, Zheng Y, Gao Y, Zhang L, Sun X, Wu Y, Zhu X, Ma F
Cell communication and signaling : CCS 2023 Sep 18;21(1):238
Cell communication and signaling : CCS 2023 Sep 18;21(1):238
Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.
Hulikova A, Park KC, Loonat AA, Gunadasa-Rohling M, Curtis MK, Chung YJ, Wilson A, Carr CA, Trafford AW, Fournier M, Moshnikova A, Andreev OA, Reshetnyak YK, Riley PR, Smart N, Milne TA, Crump NT, Swietach P
Basic research in cardiology 2022 Mar 31;117(1):17
Basic research in cardiology 2022 Mar 31;117(1):17
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human heart tissue were subjected to SDS PAGE followed by western blot with 21527-1-AP(MYLK3 antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon using 21527-1-AP(MYLK3 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The MYLK3 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human MYLK3. This antibody recognizes human antigen. The MYLK3 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
