PA5-100908

antibody from Invitrogen Antibodies

Targeting: MYLK3 caMLCK, MLCK

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-100908

Provider

Invitrogen Antibodies

Product name

MLCK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of total MYLK3.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Loss of cardiac myosin light chain kinase contributes to contractile dysfunction in right ventricular pressure overload.

Prasad V, Makkaoui N, Rajan R, Patel A, Mainali B, Bagchi P, Kumar R, Rogers J, Diamond J, Maxwell JT
Physiological reports 2022 Apr;10(7):e15238

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1 FIGURE cMLCK is downregulated in RV myocytes from PAB animals. (a) Volcano plot of proteins identified and quantified by mass spectrometry in the RV myocytes from PAB versus CTL animals (positive log2(fold change) indicates higher expression in PAB myocytes). Each point represents the difference in expression ( x -axis) between the groups and the associated significance of this change ( y -axis) by independent unpaired samples t -tests. Proteins that are significantly (+-1.3-fold, p < 0.05) upregulated (red) or downregulated (green) are identified with gene labels ( n = 4 hearts each group). Inset table shows the significantly upregulated (red) and downregulated (green) protein and the log2 (fold difference) values for each. (b) Protein expression graphs of quantified Western blot data for cMLCK in CTL RV versus PAB RV ( p < 0.001, unpaired t -test, n = 4 CTL hearts, 5 PAB hearts) and in (c) CTL LV versus CTL RV ( p < 0.001, unpaired t -test, n = 3 LV, 5 RV), and for (d) pMLC-2v in CTL versus PAB RV ( p < 0.001, unpaired t -test, n = 7 CTL, 9 PAB). (e) Real time RT-PCR mRNA expression data for mylk3 gene from CTL RV versus PAB RV ( p = 0.006, unpaired t test, n = 4 CTL, 4 PAB) and (f) CTL LV versus CTL RV ( p = 0.02, unpaired t -test, n = 4 CTL, 4 PAB). (g, h) Representative Western blots for cMLCK, MLC-2v, and pMLC-2v. Gel lanes were arranged from original blot for clarity (* p < 0.05, unpaired t -test). All N's represent individual hearts or heart tissues from individual