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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- 21527-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MYLK3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MDTKLNMLN EKVDQLLHFQ EDVTEKLQSM CRDMGHLERG LHRLEASRAP GPGGADGVPH IDTQAGWPEV LELVRAMQQD AAQHGARLEA LFRMVAAVDR AIALVGATFQ KSKVADFLMQ GRVPWRRGSP GDSPEENKER VEEEGGKPKH VLSTSGLQSD AREPGEESQK ADVLEGTAER LPPIRASGLG ADPAQAVVSP GQGDGVPGPA QAFPGHLPLP TKVEAKAPET PSENLRTGLE LAPAPGRVNV VSPSLEVAPG AGQGASSSRP DPEPLEEGTR LTPGPGPQCP GPPGLPAQAR ATHSGGETPP RISIHIQEMD TPGEMLMTGR GSLGPTLTTE APAAAQPGKQ G (1-350 aa encoded by BC109097)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.22 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human heart tissue were subjected to SDS PAGE followed by western blot with 21527-1-AP (MYLK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human heart tissue were subjected to SDS PAGE followed by western blot with 21527-1-AP (MYLK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon using 21527-1-AP (MYLK3 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon using 21527-1-AP (MYLK3 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 (under 40x lens).
