Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP14866PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP14866PU-N, RRID:AB_1769792
- Product name
- anti MLCK (N-term)
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MLCK.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- (LEFT)Western blot analysis of anti-MLCK Antibody (N-term) in A375 cell line lysates (35ug/lane). MLCK(arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of anti-MLCK Antibody (N-term) in mouse heart tissue lysates (35ug/lane). MLCK(arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- (LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MLCK antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.