HPA017303

ARPP-21, R3HDM3, TARPP

Eligible for validation within the Antibodypedia Validation Initiative

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Validation data

Product number: HPA017303
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA017303, RRID:AB_1233494
Product name: Anti-ARPP21
Provider product page: Atlas Antibodies - HPA017303
Antibody type: Polyclonal
Description: Affinity purified using the PrEST antigen as affinity ligand
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: EQGDLNQAIAEEGGTEQETATPENGIVKSESLDEE EKLELQRRLEAQNQERRKSKSGAGKGKLTRSLAVC EESSARPGGESLQDQESIHLQLSSFSSLQEEDKSR KDDSEREKEKDKNKDKTSEKPKIRMLSKDCSQEYT
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human lateral ventricle shows moderate cytoplasmic and nuclear positivity in neuronal cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human cerebral cortex shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human pancreas shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney shows no positivity in cells in tubules as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human prostate shows no positivity in glandular cells as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex shows weak cytoplasmic positivity in neurons.
Sample type: HUMAN

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-ARPP21 antibody. Corresponding ARPP21 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human cerebral cortex and prostate tissues using HPA017303 antibody. Corresponding ARPP21 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.