16757-1-AP

antibody from Proteintech Group

Targeting: GPT2 ALT2

Provider product page for 16757-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [48]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 16757-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#16757-1-AP, RRID:AB_2112098
Product name: GPT2 antibody
Antibody type: Polyclonal
Description: KD/KO validated GPT2 antibody (Cat. #16757-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

GPT2 protein structure - 16757-1-AP shown in red.

Submitted references Comprehensive Bioinformatics Analysis and Validation of the Mechanism of Glutamic-pyruvic Transaminase 2 in Bladder Cancer.

Dong H, Li H, Chen S, Wang Q, Zhang Y, Shi H, Zuo J, Chen J, Wang J, Wang H
Journal of Cancer 2025;16(15):4357-4370

Small molecule inhibition of ubiquitin C-terminal hydrolase L1 alters cell metabolism proteins and exerts anti- or pro-tumorigenic effects contingent upon chemosensitivity status in high grade serous ovarian cancer.

Jansen C, McAdams J, Kim C, De La Cruz P, Salaverria A, DaSilva NA, Grive K, James NE
Frontiers in pharmacology 2025;16:1547164

Selective Alanine Transporter Utilization Is a Therapeutic Vulnerability in ARID1A-Mutant Ovarian Cancer.

Nie H, Liao L, Zielinski RJ, Gomez JA, Basi AV, Seeley EH, Tan L, Bilecz AJ, Zhou W, Liu H, Wang C, Wu S, Qi Y, Miyamoto T, Severi F, Goldman AR, Gu S, Sood AK, Jazaeri AA, Drapkin R, Claiborne DT, Zhang N, Lorenzi PL, Burks JK, Lengyel E, Gottlieb E, Zhang R
Cancer research 2025 Sep 15;85(18):3471-3489

SLAMF7 promotes TCRαβ(+) double negative T cell antitumor activity through enhancing glutamine metabolism.

Xu N, Fang P, Zhou L, Han X, Jiang Y, Wang X, Zhu J, Li B, Zhang Z, Jin H, Du X, Sun G, Zhang D
Journal of experimental & clinical cancer research : CR 2025 Oct 30;44(1):297

MRI of ASCT2-mediated amino acid uptake in xenograft tumor models.

Ghaemi B, Krishnamachary B, Dillman N, Bains Williams KN, Hodo Y, Li Y, Penet Vidaver MF, Pomper MG, Tressler CM, Bhujwalla ZM, McMahon MT, Bulte JWM, Zijl PCM, Thomas AM
Research square 2025 Oct 26;

Elevation of hepatic de novo lipogenesis in mice with overnutrition is dependent on multiple substrates.

Strober JW, Siebel S, Murray SF, Rodríguez MG, Rodriguez-Navas Gonzalez C, Vatner DF
Journal of lipid research 2025 Jul;66(7):100838

TGF-β Coordinates Alanine Synthesis and Import for Myofibroblast Differentiation in Pulmonary Fibrosis.

Li F, Vigder N, Ziehr DR, Kamiya M, Nguyen HN, Steinhauser ML, Kim EY, Oldham WM
bioRxiv : the preprint server for biology 2025 Jul 24;

Copper Drives Remodeling of Metabolic State and Progression of Clear Cell Renal Cell Carcinoma.

Bischoff ME, Shamsaei B, Yang J, Secic D, Vemuri B, Reisz JA, D'Alessandro A, Bartolacci C, Adamczak R, Schmidt L, Wang J, Martines A, Venkat J, Tcheuyap VT, Biesiada J, Behrmann CA, Vest KE, Brugarolas J, Scaglioni PP, Plas DR, Patra KC, Gulati S, Landero Figueroa JA, Meller J, Cunningham JT, Czyzyk-Krzeska MF
Cancer discovery 2025 Feb 7;15(2):401-426

Alanine catabolism as a targetable vulnerability for MYC-driven liver cancer.

Montoya T, Lee JV, Qiu L, Krall A, Matulionis N, Seo Y, Finck BN, Kelley RK, Christofk H, Goga A
bioRxiv : the preprint server for biology 2025 Aug 12;

Imaging the uptake and metabolism of glutamine in prostate tumor models using CEST MRI.

Hodo Y, Tressler CM, Ghaemi B, Thomas R, Webster AS, Bains Williams KN, Li Y, Pomper MG, Dang CV, Bhujwalla ZM, Bulte JWM, van Zijl PCM, Thomas AM
Npj imaging 2025 Aug 1;3(1):34

Systematic profiling of mitochondria-related transcriptome in tumorigenesis, prognosis, and tumor immune microenvironment of intrahepatic cholangiocarcinoma: a multi-center cohort study.

Chen B, Lu M, Chen Q, Zou E, Bo Z, Li J, Zhao R, Zhao J, Yu Z, Chen G, Wu L
Frontiers in genetics 2024;15:1430885

Screening mitochondria-related biomarkers in skin and plasma of atopic dermatitis patients by bioinformatics analysis and machine learning.

Yu H, Lin J, Yuan J, Sun X, Wang C, Bai B
Frontiers in immunology 2024;15:1367602

Glutaminolysis provides nucleotides and amino acids to regulate osteoclast differentiation in mice.

Hu G, Yu Y, Ren Y, Tower RJ, Zhang GF, Karner CM
EMBO reports 2024 Oct;25(10):4515-4541

Loss of mitochondrial enzyme GPT2 leads to reprogramming of synaptic glutamate metabolism.

Baytas O, Davidson SM, Kauer JA, Morrow EM
bioRxiv : the preprint server for biology 2024 Nov 7;

Loss of mitochondrial enzyme GPT2 leads to reprogramming of synaptic glutamate metabolism.

Baytas O, Davidson SM, Kauer JA, Morrow EM
Molecular brain 2024 Nov 27;17(1):87

Inosine induces stemness features in CAR-T cells and enhances potency.

Klysz DD, Fowler C, Malipatlolla M, Stuani L, Freitas KA, Chen Y, Meier S, Daniel B, Sandor K, Xu P, Huang J, Labanieh L, Keerthi V, Leruste A, Bashti M, Mata-Alcazar J, Gkitsas N, Guerrero JA, Fisher C, Patel S, Asano K, Patel S, Davis KL, Satpathy AT, Feldman SA, Sotillo E, Mackall CL
Cancer cell 2024 Feb 12;42(2):266-282.e8

BET inhibition induces GDH1-dependent glutamine metabolic remodeling and vulnerability in liver cancer.

Mi W, You J, Li L, Zhu L, Xia X, Yang L, Li F, Xu Y, Bi J, Liu P, Chen L, Li F
Life metabolism 2024 Aug;3(4):loae016

The delta subunit of the GABA(A) receptor is necessary for the GPT2-promoted breast cancer metastasis.

Li N, Xu X, Liu D, Gao J, Gao Y, Wu X, Sheng H, Li Q, Mi J
Theranostics 2023;13(4):1355-1369

Loss of CD4(+) T cell-intrinsic arginase 1 accelerates Th1 response kinetics and reduces lung pathology during influenza infection.

West EE, Merle NS, Kamiński MM, Palacios G, Kumar D, Wang L, Bibby JA, Overdahl K, Jarmusch AK, Freeley S, Lee DY, Thompson JW, Yu ZX, Taylor N, Sitbon M, Green DR, Bohrer A, Mayer-Barber KD, Afzali B, Kazemian M, Scholl-Buergi S, Karall D, Huemer M, Kemper C
Immunity 2023 Sep 12;56(9):2036-2053.e12

Aspirin reprogrammes colorectal cancer cell metabolism and sensitises to glutaminase inhibition.

Holt AK, Najumudeen AK, Collard TJ, Li H, Millett LM, Hoskin AJ, Legge DN, Mortensson EMH, Flanagan DJ, Jones N, Kollareddy M, Timms P, Hitchings MD, Cronin J, Sansom OJ, Williams AC, Vincent EE
Cancer & metabolism 2023 Oct 19;11(1):18

A prognostic risk model, tumor immune environment modulation, and drug prediction of ferroptosis and amino acid metabolism-related genes in hepatocellular carcinoma.

Ji L, Zhang Q, Cao Y, Liu L
Human cell 2023 May;36(3):1173-1189

Dysregulated Gln-Glu-α-ketoglutarate axis impairs maternal decidualization and increases the risk of recurrent spontaneous miscarriage.

Tang L, Xu XH, Xu S, Liu Z, He Q, Li W, Sun J, Shuai W, Mao J, Zhao JY, Jin L
Cell reports. Medicine 2023 May 16;4(5):101026

Exosomal GPT2 derived from triple-negative breast cancer cells promotes metastasis by activating BTRC.

Cui M, Peng J, Zhou Y, Wang X, Cui D
Thoracic cancer 2023 Jul;14(21):2018-2025

Pyruvate transamination and NAD biosynthesis enable proliferation of succinate dehydrogenase-deficient cells by supporting aerobic glycolysis.

Ricci L, Stanley FU, Eberhart T, Mainini F, Sumpton D, Cardaci S
Cell death & disease 2023 Jul 6;14(7):403

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.

Southwell N, Primiano G, Nadkarni V, Attarwala N, Beattie E, Miller D, Alam S, Liparulo I, Shurubor YI, Valentino ML, Carelli V, Servidei S, Gross SS, Manfredi G, Chen Q, D'Aurelio M
EMBO molecular medicine 2023 Jul 10;15(7):e16951

Grain-Sized Moxibustion Heightens the AntiTumor Effect of Cyclophosphamide in Hepa1-6 Bearing Mice.

Zhu T, Ma Y, Wang J, Chen X, Li J, Meng L, Hou Y, Cheng Y
Evidence-based complementary and alternative medicine : eCAM 2022;2022:3684899

Thyroid hormone regulates glutamine metabolism and anaplerotic fluxes by inducing mitochondrial glutamate aminotransferase GPT2.

Cicatiello AG, Sagliocchi S, Nappi A, Di Cicco E, Miro C, Murolo M, Stornaiuolo M, Dentice M
Cell reports 2022 Mar 22;38(12):110562

Targeting BCAT1 Combined with α-Ketoglutarate Triggers Metabolic Synthetic Lethality in Glioblastoma.

Zhang B, Peng H, Zhou M, Bao L, Wang C, Cai F, Zhang H, Wang JE, Niu Y, Chen Y, Wang Y, Hatanpaa KJ, Copland JA, DeBerardinis RJ, Wang Y, Luo W
Cancer research 2022 Jul 5;82(13):2388-2402

Thyroid hormone regulates glutamine metabolism and anaplerotic fluxes by inducing mitochondrial glutamate aminotransferase GPT2.

Cicatiello AG, Sagliocchi S, Nappi A, Di Cicco E, Miro C, Murolo M, Stornaiuolo M, Dentice M
Cell reports 2022 Feb 22;38(8):110409

SPTBN1 abrogates renal clear cell carcinoma progression via glycolysis reprogramming in a GPT2-dependent manner.

Wu J, Miao C, Wang Y, Wang S, Wang Z, Liu Y, Wang X, Wang Z
Journal of translational medicine 2022 Dec 16;20(1):603

GPT2 Is Induced by Hypoxia-Inducible Factor (HIF)-2 and Promotes Glioblastoma Growth.

Zhang B, Chen Y, Bao L, Luo W
Cells 2022 Aug 20;11(16)

A Novel Ferroptosis-Based Molecular Signature Associated with Biochemical Recurrence-Free Survival and Tumor Immune Microenvironment of Prostate Cancer.

Ke ZB, You Q, Sun JB, Zhu JM, Li XD, Chen DN, Su L, Zheng QS, Wei Y, Xue XY, Xu N
Frontiers in cell and developmental biology 2021;9:774625

Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.

Zhang X, Yu K, Ma L, Qian Z, Tian X, Miao Y, Niu Y, Xu X, Guo S, Yang Y, Wang Z, Xue X, Gu C, Fang W, Sun J, Yu Y, Wang J
Theranostics 2021;11(12):5650-5674

Enhanced glutamine utilization mediated by SLC1A5 and GPT2 is an essential metabolic feature of colorectal signet ring cell carcinoma with therapeutic potential.

Wang R, Xiang W, Xu Y, Han L, Li Q, Dai W, Cai G
Annals of translational medicine 2020 Mar;8(6):302

Selective Alanine Transporter Utilization Creates a Targetable Metabolic Niche in Pancreatic Cancer.

Parker SJ, Amendola CR, Hollinshead KER, Yu Q, Yamamoto K, Encarnación-Rosado J, Rose RE, LaRue MM, Sohn ASW, Biancur DE, Paulo JA, Gygi SP, Jones DR, Wang H, Philips MR, Bar-Sagi D, Mancias JD, Kimmelman AC
Cancer discovery 2020 Jul;10(7):1018-1037

Exosome-transmitted microRNA-133b inhibited bladder cancer proliferation by upregulating dual-specificity protein phosphatase 1.

Cai X, Qu L, Yang J, Xu J, Sun L, Wei X, Qu X, Bai T, Guo Z, Zhu Y
Cancer medicine 2020 Aug;9(16):6009-6019

Deactivation of Glutaminolysis Sensitizes PIK3CA-Mutated Colorectal Cancer Cells to Aspirin-Induced Growth Inhibition.

Boku S, Watanabe M, Sukeno M, Yaoi T, Hirota K, Iizuka-Ohashi M, Itoh K, Sakai T
Cancers 2020 Apr 30;12(5)

T Cell Activation Depends on Extracellular Alanine.

Ron-Harel N, Ghergurovich JM, Notarangelo G, LaFleur MW, Tsubosaka Y, Sharpe AH, Rabinowitz JD, Haigis MC
Cell reports 2019 Sep 17;28(12):3011-3021.e4

Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.

Hamanaka RB, O'Leary EM, Witt LJ, Tian Y, Gökalp GA, Meliton AY, Dulin NO, Mutlu GM
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606

Mitochondrial GPT2 plays a pivotal role in metabolic adaptation to the perturbation of mitochondrial glutamine metabolism.

Kim M, Gwak J, Hwang S, Yang S, Jeong SM
Oncogene 2019 Jun;38(24):4729-4738

Preventing BRCA1/ZBRK1 repressor complex binding to the GOT2 promoter results in accelerated aspartate biosynthesis and promotion of cell proliferation.

Hong R, Zhang W, Xia X, Zhang K, Wang Y, Wu M, Fan J, Li J, Xia W, Xu F, Chen J, Wang S, Zhan Q
Molecular oncology 2019 Apr;13(4):959-977

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Chen Q, Kirk K, Shurubor YI, Zhao D, Arreguin AJ, Shahi I, Valsecchi F, Primiano G, Calder EL, Carelli V, Denton TT, Beal MF, Gross SS, Manfredi G, D'Aurelio M
Cell metabolism 2018 May 1;27(5):1007-1025.e5

Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.

Bharti SK, Mironchik Y, Wildes F, Penet MF, Goggins E, Krishnamachary B, Bhujwalla ZM
Oncotarget 2018 Mar 16;9(20):15326-15339

Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Wang YQ, Wang HL, Xu J, Tan J, Fu LN, Wang JL, Zou TH, Sun DF, Gao QY, Chen YX, Fang JY
Nature communications 2018 Feb 7;9(1):545

Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

Cai WF, Zhang C, Wu YQ, Zhuang G, Ye Z, Zhang CS, Lin SC
Cell research 2018 Aug;28(8):865-867

Glutamic Pyruvate Transaminase GPT2 Promotes Tumorigenesis of Breast Cancer Cells by Activating Sonic Hedgehog Signaling.

Cao Y, Lin SH, Wang Y, Chin YE, Kang L, Mi J
Theranostics 2017;7(12):3021-3033

Mutations in mitochondrial enzyme GPT2 cause metabolic dysfunction and neurological disease with developmental and progressive features.

Ouyang Q, Nakayama T, Baytas O, Davidson SM, Yang C, Schmidt M, Lizarraga SB, Mishra S, Ei-Quessny M, Niaz S, Gul Butt M, Imran Murtaza S, Javed A, Chaudhry HR, Vaughan DJ, Hill RS, Partlow JN, Yoo SY, Lam AT, Nasir R, Al-Saffar M, Barkovich AJ, Schwede M, Nagpal S, Rajab A, DeBerardinis RJ, Housman DE, Mochida GH, Morrow EM
Proceedings of the National Academy of Sciences of the United States of America 2016 Sep 20;113(38):E5598-607

Transcriptomic and metabolic analyses reveal salvage pathways in creatine-deficient AGAT(-/-) mice.

Stockebrand M, Nejad AS, Neu A, Kharbanda KK, Sauter K, Schillemeit S, Isbrandt D, Choe CU
Amino acids 2016 Aug;48(8):2025-39

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16757-1-AP( GPT2 Antibody) at dilution of 1:600
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human kidney using 16757-1-AP(GPT2 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The GPT2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human GPT2. This antibody recognizes human,mouse,rat antigen. The GPT2 antibody has been validated for the following applications: ELISA, IHC, WB analysis.
Appendix: 16757-1-AP.pdf

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