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- Antigen structure
- References [30]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 16757-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#16757-1-AP, RRID:AB_2112098
- Product name
- GPT2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated GPT2 antibody (Cat. #16757-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Inosine induces stemness features in CAR-T cells and enhances potency.
The delta subunit of the GABA(A) receptor is necessary for the GPT2-promoted breast cancer metastasis.
Loss of CD4(+) T cell-intrinsic arginase 1 accelerates Th1 response kinetics and reduces lung pathology during influenza infection.
Aspirin reprogrammes colorectal cancer cell metabolism and sensitises to glutaminase inhibition.
A prognostic risk model, tumor immune environment modulation, and drug prediction of ferroptosis and amino acid metabolism-related genes in hepatocellular carcinoma.
Dysregulated Gln-Glu-α-ketoglutarate axis impairs maternal decidualization and increases the risk of recurrent spontaneous miscarriage.
Exosomal GPT2 derived from triple-negative breast cancer cells promotes metastasis by activating BTRC.
Pyruvate transamination and NAD biosynthesis enable proliferation of succinate dehydrogenase-deficient cells by supporting aerobic glycolysis.
A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.
Thyroid hormone regulates glutamine metabolism and anaplerotic fluxes by inducing mitochondrial glutamate aminotransferase GPT2.
Targeting BCAT1 Combined with α-Ketoglutarate Triggers Metabolic Synthetic Lethality in Glioblastoma.
Thyroid hormone regulates glutamine metabolism and anaplerotic fluxes by inducing mitochondrial glutamate aminotransferase GPT2.
SPTBN1 abrogates renal clear cell carcinoma progression via glycolysis reprogramming in a GPT2-dependent manner.
GPT2 Is Induced by Hypoxia-Inducible Factor (HIF)-2 and Promotes Glioblastoma Growth.
A Novel Ferroptosis-Based Molecular Signature Associated with Biochemical Recurrence-Free Survival and Tumor Immune Microenvironment of Prostate Cancer.
Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.
Enhanced glutamine utilization mediated by SLC1A5 and GPT2 is an essential metabolic feature of colorectal signet ring cell carcinoma with therapeutic potential.
Exosome-transmitted microRNA-133b inhibited bladder cancer proliferation by upregulating dual-specificity protein phosphatase 1.
Deactivation of Glutaminolysis Sensitizes PIK3CA-Mutated Colorectal Cancer Cells to Aspirin-Induced Growth Inhibition.
T Cell Activation Depends on Extracellular Alanine.
Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.
Mitochondrial GPT2 plays a pivotal role in metabolic adaptation to the perturbation of mitochondrial glutamine metabolism.
Preventing BRCA1/ZBRK1 repressor complex binding to the GOT2 promoter results in accelerated aspartate biosynthesis and promotion of cell proliferation.
Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.
Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.
Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.
Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.
Glutamic Pyruvate Transaminase GPT2 Promotes Tumorigenesis of Breast Cancer Cells by Activating Sonic Hedgehog Signaling.
Mutations in mitochondrial enzyme GPT2 cause metabolic dysfunction and neurological disease with developmental and progressive features.
Transcriptomic and metabolic analyses reveal salvage pathways in creatine-deficient AGAT(-/-) mice.
Klysz DD, Fowler C, Malipatlolla M, Stuani L, Freitas KA, Chen Y, Meier S, Daniel B, Sandor K, Xu P, Huang J, Labanieh L, Keerthi V, Leruste A, Bashti M, Mata-Alcazar J, Gkitsas N, Guerrero JA, Fisher C, Patel S, Asano K, Patel S, Davis KL, Satpathy AT, Feldman SA, Sotillo E, Mackall CL
Cancer cell 2024 Feb 12;42(2):266-282.e8
Cancer cell 2024 Feb 12;42(2):266-282.e8
The delta subunit of the GABA(A) receptor is necessary for the GPT2-promoted breast cancer metastasis.
Li N, Xu X, Liu D, Gao J, Gao Y, Wu X, Sheng H, Li Q, Mi J
Theranostics 2023;13(4):1355-1369
Theranostics 2023;13(4):1355-1369
Loss of CD4(+) T cell-intrinsic arginase 1 accelerates Th1 response kinetics and reduces lung pathology during influenza infection.
West EE, Merle NS, Kamiński MM, Palacios G, Kumar D, Wang L, Bibby JA, Overdahl K, Jarmusch AK, Freeley S, Lee DY, Thompson JW, Yu ZX, Taylor N, Sitbon M, Green DR, Bohrer A, Mayer-Barber KD, Afzali B, Kazemian M, Scholl-Buergi S, Karall D, Huemer M, Kemper C
Immunity 2023 Sep 12;56(9):2036-2053.e12
Immunity 2023 Sep 12;56(9):2036-2053.e12
Aspirin reprogrammes colorectal cancer cell metabolism and sensitises to glutaminase inhibition.
Holt AK, Najumudeen AK, Collard TJ, Li H, Millett LM, Hoskin AJ, Legge DN, Mortensson EMH, Flanagan DJ, Jones N, Kollareddy M, Timms P, Hitchings MD, Cronin J, Sansom OJ, Williams AC, Vincent EE
Cancer & metabolism 2023 Oct 19;11(1):18
Cancer & metabolism 2023 Oct 19;11(1):18
A prognostic risk model, tumor immune environment modulation, and drug prediction of ferroptosis and amino acid metabolism-related genes in hepatocellular carcinoma.
Ji L, Zhang Q, Cao Y, Liu L
Human cell 2023 May;36(3):1173-1189
Human cell 2023 May;36(3):1173-1189
Dysregulated Gln-Glu-α-ketoglutarate axis impairs maternal decidualization and increases the risk of recurrent spontaneous miscarriage.
Tang L, Xu XH, Xu S, Liu Z, He Q, Li W, Sun J, Shuai W, Mao J, Zhao JY, Jin L
Cell reports. Medicine 2023 May 16;4(5):101026
Cell reports. Medicine 2023 May 16;4(5):101026
Exosomal GPT2 derived from triple-negative breast cancer cells promotes metastasis by activating BTRC.
Cui M, Peng J, Zhou Y, Wang X, Cui D
Thoracic cancer 2023 Jul;14(21):2018-2025
Thoracic cancer 2023 Jul;14(21):2018-2025
Pyruvate transamination and NAD biosynthesis enable proliferation of succinate dehydrogenase-deficient cells by supporting aerobic glycolysis.
Ricci L, Stanley FU, Eberhart T, Mainini F, Sumpton D, Cardaci S
Cell death & disease 2023 Jul 6;14(7):403
Cell death & disease 2023 Jul 6;14(7):403
A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.
Southwell N, Primiano G, Nadkarni V, Attarwala N, Beattie E, Miller D, Alam S, Liparulo I, Shurubor YI, Valentino ML, Carelli V, Servidei S, Gross SS, Manfredi G, Chen Q, D'Aurelio M
EMBO molecular medicine 2023 Jul 10;15(7):e16951
EMBO molecular medicine 2023 Jul 10;15(7):e16951
Thyroid hormone regulates glutamine metabolism and anaplerotic fluxes by inducing mitochondrial glutamate aminotransferase GPT2.
Cicatiello AG, Sagliocchi S, Nappi A, Di Cicco E, Miro C, Murolo M, Stornaiuolo M, Dentice M
Cell reports 2022 Mar 22;38(12):110562
Cell reports 2022 Mar 22;38(12):110562
Targeting BCAT1 Combined with α-Ketoglutarate Triggers Metabolic Synthetic Lethality in Glioblastoma.
Zhang B, Peng H, Zhou M, Bao L, Wang C, Cai F, Zhang H, Wang JE, Niu Y, Chen Y, Wang Y, Hatanpaa KJ, Copland JA, DeBerardinis RJ, Wang Y, Luo W
Cancer research 2022 Jul 5;82(13):2388-2402
Cancer research 2022 Jul 5;82(13):2388-2402
Thyroid hormone regulates glutamine metabolism and anaplerotic fluxes by inducing mitochondrial glutamate aminotransferase GPT2.
Cicatiello AG, Sagliocchi S, Nappi A, Di Cicco E, Miro C, Murolo M, Stornaiuolo M, Dentice M
Cell reports 2022 Feb 22;38(8):110409
Cell reports 2022 Feb 22;38(8):110409
SPTBN1 abrogates renal clear cell carcinoma progression via glycolysis reprogramming in a GPT2-dependent manner.
Wu J, Miao C, Wang Y, Wang S, Wang Z, Liu Y, Wang X, Wang Z
Journal of translational medicine 2022 Dec 16;20(1):603
Journal of translational medicine 2022 Dec 16;20(1):603
GPT2 Is Induced by Hypoxia-Inducible Factor (HIF)-2 and Promotes Glioblastoma Growth.
Zhang B, Chen Y, Bao L, Luo W
Cells 2022 Aug 20;11(16)
Cells 2022 Aug 20;11(16)
A Novel Ferroptosis-Based Molecular Signature Associated with Biochemical Recurrence-Free Survival and Tumor Immune Microenvironment of Prostate Cancer.
Ke ZB, You Q, Sun JB, Zhu JM, Li XD, Chen DN, Su L, Zheng QS, Wei Y, Xue XY, Xu N
Frontiers in cell and developmental biology 2021;9:774625
Frontiers in cell and developmental biology 2021;9:774625
Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.
Zhang X, Yu K, Ma L, Qian Z, Tian X, Miao Y, Niu Y, Xu X, Guo S, Yang Y, Wang Z, Xue X, Gu C, Fang W, Sun J, Yu Y, Wang J
Theranostics 2021;11(12):5650-5674
Theranostics 2021;11(12):5650-5674
Enhanced glutamine utilization mediated by SLC1A5 and GPT2 is an essential metabolic feature of colorectal signet ring cell carcinoma with therapeutic potential.
Wang R, Xiang W, Xu Y, Han L, Li Q, Dai W, Cai G
Annals of translational medicine 2020 Mar;8(6):302
Annals of translational medicine 2020 Mar;8(6):302
Exosome-transmitted microRNA-133b inhibited bladder cancer proliferation by upregulating dual-specificity protein phosphatase 1.
Cai X, Qu L, Yang J, Xu J, Sun L, Wei X, Qu X, Bai T, Guo Z, Zhu Y
Cancer medicine 2020 Aug;9(16):6009-6019
Cancer medicine 2020 Aug;9(16):6009-6019
Deactivation of Glutaminolysis Sensitizes PIK3CA-Mutated Colorectal Cancer Cells to Aspirin-Induced Growth Inhibition.
Boku S, Watanabe M, Sukeno M, Yaoi T, Hirota K, Iizuka-Ohashi M, Itoh K, Sakai T
Cancers 2020 Apr 30;12(5)
Cancers 2020 Apr 30;12(5)
T Cell Activation Depends on Extracellular Alanine.
Ron-Harel N, Ghergurovich JM, Notarangelo G, LaFleur MW, Tsubosaka Y, Sharpe AH, Rabinowitz JD, Haigis MC
Cell reports 2019 Sep 17;28(12):3011-3021.e4
Cell reports 2019 Sep 17;28(12):3011-3021.e4
Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.
Hamanaka RB, O'Leary EM, Witt LJ, Tian Y, Gökalp GA, Meliton AY, Dulin NO, Mutlu GM
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606
Mitochondrial GPT2 plays a pivotal role in metabolic adaptation to the perturbation of mitochondrial glutamine metabolism.
Kim M, Gwak J, Hwang S, Yang S, Jeong SM
Oncogene 2019 Jun;38(24):4729-4738
Oncogene 2019 Jun;38(24):4729-4738
Preventing BRCA1/ZBRK1 repressor complex binding to the GOT2 promoter results in accelerated aspartate biosynthesis and promotion of cell proliferation.
Hong R, Zhang W, Xia X, Zhang K, Wang Y, Wu M, Fan J, Li J, Xia W, Xu F, Chen J, Wang S, Zhan Q
Molecular oncology 2019 Apr;13(4):959-977
Molecular oncology 2019 Apr;13(4):959-977
Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.
Chen Q, Kirk K, Shurubor YI, Zhao D, Arreguin AJ, Shahi I, Valsecchi F, Primiano G, Calder EL, Carelli V, Denton TT, Beal MF, Gross SS, Manfredi G, D'Aurelio M
Cell metabolism 2018 May 1;27(5):1007-1025.e5
Cell metabolism 2018 May 1;27(5):1007-1025.e5
Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.
Bharti SK, Mironchik Y, Wildes F, Penet MF, Goggins E, Krishnamachary B, Bhujwalla ZM
Oncotarget 2018 Mar 16;9(20):15326-15339
Oncotarget 2018 Mar 16;9(20):15326-15339
Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.
Wang YQ, Wang HL, Xu J, Tan J, Fu LN, Wang JL, Zou TH, Sun DF, Gao QY, Chen YX, Fang JY
Nature communications 2018 Feb 7;9(1):545
Nature communications 2018 Feb 7;9(1):545
Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.
Cai WF, Zhang C, Wu YQ, Zhuang G, Ye Z, Zhang CS, Lin SC
Cell research 2018 Aug;28(8):865-867
Cell research 2018 Aug;28(8):865-867
Glutamic Pyruvate Transaminase GPT2 Promotes Tumorigenesis of Breast Cancer Cells by Activating Sonic Hedgehog Signaling.
Cao Y, Lin SH, Wang Y, Chin YE, Kang L, Mi J
Theranostics 2017;7(12):3021-3033
Theranostics 2017;7(12):3021-3033
Mutations in mitochondrial enzyme GPT2 cause metabolic dysfunction and neurological disease with developmental and progressive features.
Ouyang Q, Nakayama T, Baytas O, Davidson SM, Yang C, Schmidt M, Lizarraga SB, Mishra S, Ei-Quessny M, Niaz S, Gul Butt M, Imran Murtaza S, Javed A, Chaudhry HR, Vaughan DJ, Hill RS, Partlow JN, Yoo SY, Lam AT, Nasir R, Al-Saffar M, Barkovich AJ, Schwede M, Nagpal S, Rajab A, DeBerardinis RJ, Housman DE, Mochida GH, Morrow EM
Proceedings of the National Academy of Sciences of the United States of America 2016 Sep 20;113(38):E5598-607
Proceedings of the National Academy of Sciences of the United States of America 2016 Sep 20;113(38):E5598-607
Transcriptomic and metabolic analyses reveal salvage pathways in creatine-deficient AGAT(-/-) mice.
Stockebrand M, Nejad AS, Neu A, Kharbanda KK, Sauter K, Schillemeit S, Isbrandt D, Choe CU
Amino acids 2016 Aug;48(8):2025-39
Amino acids 2016 Aug;48(8):2025-39
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16757-1-AP( GPT2 Antibody) at dilution of 1:600
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 16757-1-AP(GPT2 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The GPT2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human GPT2. This antibody recognizes human,mouse,rat antigen. The GPT2 antibody has been validated for the following applications: ELISA, IHC, WB analysis.
