Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C744795 - Provider product page
- Provider
- LSBio
- Product name
- PGA3 Antibody LS-C744795
- Antibody type
- Polyclonal
- Reactivity
- Porcine
- Host
- Goat
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western Blot of Goat anti-Pepsin Antibody. Lane 1: Pepsin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Pepsin primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 35 kDa, 35 kDa for Pepsin. Other band(s): None.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of goat anti-pepsin antibody Antigen Retrieval: HIER pH6.2 10X Primary Antibody: Goat Anti-Pepsin 1 hr 1:500 Secondary Antibody: Swine anti-goat HRP 45 min followed by Staining with DAB. Staining: Gastric mucosa (containing fundic gland with zymogenic cells) homogeneus positivity in the secretory granules of glandular chief cells and in secretory granular of neck cells (Shown Left and Center) negative control with normal rabbit IgG (Shown right).