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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA5-120544 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PGA3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: C6, U-251MG, U-87MG, SGC-7901, Mouse brain, Rat brain. The target is usually found in the following locations: Secreted. Immunogen sequence: VDEQPLENYL DMEYFGTIGI GTPAQDFTVV FDTGSSNLWV PSVYCSSLAC TNHNRFNPED SSTYQSTSET VSITYGTGSM TGILGYDTVQ VGGISDTNQI FGLSETEPGS FLYYAPFD
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 2.53 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of PGA3 in extracts of various cell lines. Samples were incubated with PGA3 Polyclonal antibody (Product # PA5-120544) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
