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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-29142 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- gamma Synuclein Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLa, A431.
- Concentration
- 0.83 mg/mL
Submitted references Chimera RNA interference knockdown of γ-synuclein in human cortical astrocytes results in mitotic catastrophe.
Le T, Winham CL, Andromidas F, Silver AC, Jellison ER, Levesque AA, Koob AO
Neural regeneration research 2020 Oct;15(10):1894-1902
Neural regeneration research 2020 Oct;15(10):1894-1902
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of gamma Synuclein using 30 µg of A431 lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a gamma Synuclein polyclonal antibody (Product # PA5-29142) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of gamma Synuclein in paraformaldehyde-fixed HeLa cells using a gamma Synuclein polyclonal antibody (Product # PA5-29142) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- gamma Synuclein Polyclonal Antibody detects gamma Synuclein protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Gamma Synuclein Polyclonal Antibody (Product # PA5-29142) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
