Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1447 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse PDGF-C Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse PDGF-C in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human (rh) PDGF-CC is observed and less than 1% cross-reactivity with rhPDGF-AA, rhPDGF-BB, rhPDGF-D, and recombinant rat PDGF-AB is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q8CI19- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently.
Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation.
PDGF-C mediates the angiogenic and tumorigenic properties of fibroblasts associated with tumors refractory to anti-VEGF treatment.
Platelet-derived growth factor isoform expression in carbon tetrachloride-induced chronic liver injury.
Zheng L, Zhao C, Du Y, Lin X, Jiang Y, Lee C, Tian G, Mi J, Li X, Chen Q, Ye Z, Huang L, Wang S, Ren X, Xing L, Chen W, Huang D, Gao Z, Zhang S, Lu W, Tang Z, Wang B, Ju R, Li X
Oncotarget 2016 Nov 22;7(47):77902-77915
Oncotarget 2016 Nov 22;7(47):77902-77915
Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation.
Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X
The Journal of experimental medicine 2010 Apr 12;207(4):867-80
The Journal of experimental medicine 2010 Apr 12;207(4):867-80
PDGF-C mediates the angiogenic and tumorigenic properties of fibroblasts associated with tumors refractory to anti-VEGF treatment.
Crawford Y, Kasman I, Yu L, Zhong C, Wu X, Modrusan Z, Kaminker J, Ferrara N
Cancer cell 2009 Jan 6;15(1):21-34
Cancer cell 2009 Jan 6;15(1):21-34
Platelet-derived growth factor isoform expression in carbon tetrachloride-induced chronic liver injury.
Borkham-Kamphorst E, Kovalenko E, van Roeyen CR, Gassler N, Bomble M, Ostendorf T, Floege J, Gressner AM, Weiskirchen R
Laboratory investigation; a journal of technical methods and pathology 2008 Oct;88(10):1090-100
Laboratory investigation; a journal of technical methods and pathology 2008 Oct;88(10):1090-100
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by PDGF-CC and Neutralization by Mouse PDGF-C Antibody. Recombinant Mouse PDGF-CC stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by 0.8 µg/mL Recombinant Mouse PDGF-CC is neutralized (green line) by increasing concentrations of Goat Anti-Mouse PDGF-C Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF1447). The ND50 is typically 4-16 µg/mL.
