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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-47156 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDGF-C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In Western blots, approximately 15% cross-reactivity with recombinant mouse PDGF-C is observed, and less than 1% cross-reactivity with human PDGF, recombinant human (rh) PDGF-AA, rhPDGF-BB, rhPDGF-AB, and rhPDGF-D is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-human PDGF-C Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47156) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. Specific bands were detected for PDGF-C p80 at approximately 80 kDa and PDGF-C p55 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDGF-C in human platelets. Samples were incubated in PDGF-C polyclonal antibody (Product # PA5-47156) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. Specific bands were detected for PDGF‚C p80 at approximately 80 kDa and PDGF‚C p55 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDGF-C in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue. Samples were incubated in PDGF-C polyclonal antibody (Product # PA5-47156) using a dilution of 1.7 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of PDGF-C in NR6R‚3T3 mouse fibroblast cell line. Samples were incubated in PDGF-C polyclonal antibody (Product # PA5-47156). Recombinant Human PDGF‚CC stimulates proliferation in the NR6R‚3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF‚CC (0.8 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 6-24 µg/mL.
