PA5-47156

antibody from Invitrogen Antibodies

Targeting: PDGFC fallotein, SCDGF

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Blocking/Neutralizing (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47156

Provider

Invitrogen Antibodies

Product name

PDGF-C Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In Western blots, approximately 15% cross-reactivity with recombinant mouse PDGF-C is observed, and less than 1% cross-reactivity with human PDGF, recombinant human (rh) PDGF-AA, rhPDGF-BB, rhPDGF-AB, and rhPDGF-D is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of PDGF-C in NR6R‚3T3 mouse fibroblast cell line. Samples were incubated in PDGF-C polyclonal antibody (Product # PA5-47156). Recombinant Human PDGF‚CC stimulates proliferation in the NR6R‚3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF‚CC (0.8 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 6-24 µg/mL.