AF1560

antibody from R&D Systems

Targeting: PDGFC fallotein, SCDGF

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1560

Provider

R&D Systems

Product name

Human PDGF-C Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human PDGF-C in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse PDGF-C is observed, and less than 1% cross-reactivity with human PDGF, recombinant human (rh) PDGF-AA, rhPDGF-BB, rhPDGF-AB, and rhPDGF-D is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q9NRA1


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently.

Zheng L, Zhao C, Du Y, Lin X, Jiang Y, Lee C, Tian G, Mi J, Li X, Chen Q, Ye Z, Huang L, Wang S, Ren X, Xing L, Chen W, Huang D, Gao Z, Zhang S, Lu W, Tang Z, Wang B, Ju R, Li X
Oncotarget 2016 Nov 22;7(47):77902-77915

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Platelet-derived growth factor C and calpain-3 are modulators of human melanoma cell invasiveness.

Ruffini F, Tentori L, Dorio AS, Arcelli D, D'Amati G, D'Atri S, Graziani G, Lacal PM
Oncology reports 2013 Dec;30(6):2887-96

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Effects of PDGF-C and PDGF-D on monocyte migration and MMP-2 and MMP-9 expression.

Wågsäter D, Zhu C, Björck HM, Eriksson P
Atherosclerosis 2009 Feb;202(2):415-23

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human PDGF-C by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for PDGF-C p80 at approximately 80 kDa and PDGF-C p55 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

PDGF-C in Human Pancreatic Cancer Tissue. PDGF-C was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using 1.7 µg/mL Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cell Proliferation Induced by PDGF-CC and Neutralization by Human PDGF-C Antibody. Recombinant Human PDGF-CC (Catalog # 1687-CC) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-CC (0.8 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560). The ND₅₀ is typically 6-24 µg/mL.