Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX629886 - Provider product page
- Provider
- GeneTex
- Product name
- PUF60 antibody [GT677]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- PUF60 antibody [GT677] detects PUF60 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extractC. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extract7.5% SDS-PAGEPUF60 antibody [GT677] (GTX629886) dilution: 1:5000The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- PUF60 antibody [GT677] detects PUF60 protein by western blot analysis.A. 50 ?g mouse brain lysate/extract7.5% SDS-PAGEPUF60 antibody [GT677] (GTX629886) dilution: 1:1000The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PUF60 antibody [GT677] detects PUF60 protein by western blot analysis.A. 50 ?g rat brain lysate/extract7.5% SDS-PAGEPUF60 antibody [GT677] (GTX629886) dilution: 1:1000The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PUF60 antibody [GT677]PUF60 antibody [GT677] detects PUF60 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PUF60 protein stained by PUF60 antibody [GT677] (GTX629886) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- PUF60 antibody [GT677] detects PUF60 protein at on gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded AGS xenograft. PUF60 antibody [GT677] (GTX629886) dilution: 1:100.