PA5-121029

antibody from Invitrogen Antibodies

Targeting: PUF60 FIR, RoBPI, SIAHBP1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-121029

Provider

Invitrogen Antibodies

Product name

PUF60 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: THP-1, A-549, Mouse brain, Mouse thymus, Mouse spleen, Rat lung, Rat liver, Rat heart. The target is usually found in the following locations: Nucleus. Immunogen sequence: DPTTGKHKGY GFIEYEKAQS SQDAVSSMNL FDLGGQYLRV GKAVTPPMPL LTPATPGGLP PAAAVAAAAA TAKITAQEAV AGAAVLGTLG TPGLVSPALT LAQPLGTLPQ AVMAAQAPGV ITGVTPARPP IPVTIPSVGV VNPILASPPT LGLLEPKKEK EEEELFPESE RPEMLSEQEH MSISGSSARH MVMQKLLRKQ ESTVMVLRNM VDPKDIDDDL EGEVTEECGK FGAVNRVIIY QEKQGEEEDA EIIVKIFVEF SIASETHKAI QALNGRWFAG RKVVAEVYDQ ERFDNSDLSA

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.8 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PUF60 in extracts of various cell lines. Samples were incubated with PUF60 Polyclonal antibody (Product # PA5-121029) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of PUF60 in HeLa cells using a PUF60 Polyclonal antibody (Product # PA5-121029). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of PUF60 in 150 μg extracts of A549 cells. Samples were precipitated with 3 μg PUF60 Polyclonal antibody (Product # PA5-121029). Western blot was performed from the immunoprecipitate using PUF60 Polyclonal antibody (Product # PA5-121029) at a dilution of 1:500.