Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- PA5-121029 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PUF60 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: THP-1, A-549, Mouse brain, Mouse thymus, Mouse spleen, Rat lung, Rat liver, Rat heart. The target is usually found in the following locations: Nucleus. Immunogen sequence: DPTTGKHKGY GFIEYEKAQS SQDAVSSMNL FDLGGQYLRV GKAVTPPMPL LTPATPGGLP PAAAVAAAAA TAKITAQEAV AGAAVLGTLG TPGLVSPALT LAQPLGTLPQ AVMAAQAPGV ITGVTPARPP IPVTIPSVGV VNPILASPPT LGLLEPKKEK EEEELFPESE RPEMLSEQEH MSISGSSARH MVMQKLLRKQ ESTVMVLRNM VDPKDIDDDL EGEVTEECGK FGAVNRVIIY QEKQGEEEDA EIIVKIFVEF SIASETHKAI QALNGRWFAG RKVVAEVYDQ ERFDNSDLSA
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.8 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PUF60 in extracts of various cell lines. Samples were incubated with PUF60 Polyclonal antibody (Product # PA5-121029) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of PUF60 in HeLa cells using a PUF60 Polyclonal antibody (Product # PA5-121029). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of PUF60 in 150 μg extracts of A549 cells. Samples were precipitated with 3 μg PUF60 Polyclonal antibody (Product # PA5-121029). Western blot was performed from the immunoprecipitate using PUF60 Polyclonal antibody (Product # PA5-121029) at a dilution of 1:500.