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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-27124 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calcium Channel beta-4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, HepG2, A375, mouse cerebellum. Predicted reactivity: Pig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Calcium Channel beta-4 Polyclonal Antibody (Product # PA5-27124). Sample (50 µg of whole cell lysate). Lane A: mouse cerebellum . 7.5% SDS PAGE. Calcium Channel beta-4 Polyclonal Antibody (Product # PA5-27124) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Calcium Channel beta-4 Polyclonal Antibody (Product # PA5-27124). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: HepG2. Lane C: A375 . 7.5% SDS PAGE. Calcium Channel beta-4 Polyclonal Antibody (Product # PA5-27124) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CACNB4 antibody [C3], C-term detects CACNB4 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CACNB4 protein stained by CACNB4 antibody [C3], C-term (Product # PA5-27124) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of Calcium-Channel-beta-4 was performed in frozen-sectioned adult mouse cerebellum tissue using Calcium Channel beta-4 Polyclonal Antibody (Product # PA5-27124) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using CACNB4 (Product # PA5-27124) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
