AP10176b

antibody from Abgent

Targeting: UBA52 CEP52, HUBCEP52, L40, MGC126879, MGC126881, MGC57125, RPL40

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP10176b

Provider

Abgent

Proper citation

Abgent Cat#AP10176b, RRID:AB_10821906

Product name

UBA52 Antibody (C-Term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

UBA52 Antibody (C-Term) (Cat. #AP10176b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UBA52 antibody detected the UBA52 protein (arrow).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

UBA52 antibody (C-Term) (Cat. #AP10176b) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the UBA52 antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

UBA52 Antibody (C-Term) (Cat. #AP10176b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50