LS-C287223

antibody from LSBio

Targeting: UBA52 CEP52, HUBCEP52, L40, MGC126879, MGC126881, MGC57125, RPL40

Western blot (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C287223

Provider

LSBio

Product name

UBA52 Antibody (aa1-50) LS-C287223

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Bovine

Host

Rabbit

Isotype

IgG

Storage

Store at 2-8°C for up to one year.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Ubiquitin by Western Blotting of Products from an Ubiquitination Assay and Purified Ubiquitin. Samples: A) Ubiquitination assay was performed as described in Zhang et al., 2004, Mol. Cell. Biol. 24(24):10941-10953. B) Purified ubiquitin applied in decreasing amounts from 5 to 0.1 ug. Antibody: Affinity purified rabbit anti-Ubiquitin antibody used at 0.4 ug/ml for Western Blot. Detection: Alkaline phosphatase conjugated secondary antibody and development with NBT/BCIP for 10 minutes (A and B).

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human and Mouse Ubiquitin by Immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-Ubiquitin used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.