PA5-77775

antibody from Invitrogen Antibodies

Targeting: IRGM IFI1, IRGM1, LRG-47, LRG47

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-77775

Provider

Invitrogen Antibodies

Product name

IRGM Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of IRGM in human HeLa and 293T cell lysates with 15 µg of sample. The sample was blocked with 5% skim milk in TBST, incubated with IRGM polyclonal antibody (Product # PA5-77775) using a dilution of 1:1000 (2 hours at 4°C), followed by Goat Anti-Rabbit HRP (1 hour at RT) at a dilution of 1:1000 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1) Molecular Weight Ladder (MW), Lane 2) HeLa cell lysates, Lane 3) 293Trap cell lysates.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of IRGM in human colon carcinoma cell line (RKO). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with IRGM polyclonal antibody (Product # PA5-77775) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Rabbit 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: A) DAPI nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) IRGM Antibody, D) Merged image. Magnification: 60x.