Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN950299 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-ATP/GTP Binding Protein-Like 5 (AGBL5) (N-Term), (AA 106-135) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 106-135 amino acids from the N-terminal region of human AGBL5
- Description
- Affinity chromatography on Protein A
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- N-Term,AA 106-135
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags.
Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing.
Wang AG, Yoon SY, Oh JH, Jeon YJ, Kim M, Kim JM, Byun SS, Yang JO, Kim JH, Kim DG, Yeom YI, Yoo HS, Kim YS, Kim NS
Biochemical and biophysical research communications 2006 Jul 7;345(3):1022-32
Biochemical and biophysical research communications 2006 Jul 7;345(3):1022-32
Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing.
Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S
EMBO reports 2000 Sep;1(3):287-92
EMBO reports 2000 Sep;1(3):287-92
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AGBL5 Antibody (N-term) (Cat. #AP50109PU-N) western blot analysis in K562 cell line lysates (35μg/lane).This demonstrates the AGBL5 antibody detected the AGBL5 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of AGBL5 Antibody (N-term)(Cat#AP50109PU-N) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AGBL5 Antibody (N-term) (Cat. #AP50109PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AGBL5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.