720358

antibody from Invitrogen Antibodies

Targeting: DRD1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

720358

Provider

Invitrogen Antibodies

Product name

DRD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK 293 cells were transiently transfected with Dopamine Receptor D1 or Dopamine Receptor D5. After 48 h, cells were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads. Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. Membranes were then incubated with rabbit polyclonal Anti-Dopamine Receptor D1 Rabbit Polyclonal Antibody (Product # 720358 at a concentration of 2 µg/mL). Bands from 70-100 kDa corresponding to Dopamine receptor D1 was observed.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sections of human cerebral cortex were dewaxed, microwaved in citric acid and incubated with Anti-Dopamine Receptor D1 Rabbit Polyclonal Antibody (Product # 720358 at a concentration of 2 µg/mL). Sections were then sequentially treated with biotinylated anti-rabbit IgG and AB solution. Sections were then developed in AEC and lightly counterstained with hematoxylin. Inset, for adsorption controls the antibody was incubated with 10 µg/mL of the peptide used for immunizations (+ Peptide). Note, staining of neuronal somata and dendrites is completely neutralized by preincubation of the antibody with its immunizing peptide.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sections of human cerebral cortex were dewaxed, microwaved in citric acid and incubated with Anti-Dopamine Receptor D1 Rabbit Polyclonal Antibody (Product # 720358 at a concentration of 2 µg/mL). Sections were then sequentially treated with biotinylated anti-rabbit IgG and AB solution. Sections were then developed in AEC and lightly counterstained with hematoxylin. Inset, for adsorption controls the antibody was incubated with 10 µg/mL of the peptide used for immunizations (+ Peptide). Note, staining of neuronal somata and dendrites is completely neutralized by preincubation of the antibody with its immunizing peptide.