702593

antibody from Invitrogen Antibodies

Targeting: DRD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

702593

Provider

Invitrogen Antibodies

Product name

DRD1 Recombinant Rabbit Monoclonal Antibody (1H8L2)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Cat, Bovine.

Antibody clone number

1H8L2

Concentration

0.5 mg/mL

References

Submitted references

Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species.

He X, Chen W, Liu Z, Yu G, Chen Y, Cai YJ, Sun L, Xu W, Zhong L, Gao C, Chen J, Zhang M, Yang S, Yao Y, Zhang Z, Ma F, Zhang CC, Lu HP, Yu B, Cheng TL, Qiu J, Sheng Q, Zhou HM, Lv ZR, Yan J, Zhou Y, Qiu Z, Cui Z, Zhang X, Meng A, Sun Q, Yang Y
Nucleic acids research 2020 Jun 4;48(10):e57

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on HEK-293 cells stably transfected with human DRD1 (Lane 2) and Mock (Lane 1). Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. The blot was probed with Anti-DRD1 Recombinant Rabbit Monoclonal Antibody (Product # 702593, 1 µg/mL, 1:500 dilution). An ~70-100 kDa band corresponding to DRD1 was observed in the transfected cell line alone.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, HEK-293 cells were stably transfected with human DRD1. After 24hr of transfection the cells were fixed and permeabilized for detection of endogenous DRD1 using Anti- DRD1 Recombinant Rabbit Monoclonal Antibody (Product # 702593, 1 µg/mL, 1:500 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate. Panel a) shows representative cells that were stained for detection of membrane localization of the DRD1 on the transfected cells. The images were captured at 40X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunocytochemistry analysis, HEK 293 cells stably transfected with Dopamine D1 receptor (D1R) were grown on poly-L-lysine-coated coverslips overnight. Cells were then treated with 10 µM dopamine for 30 min, washed with PBS and fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti- DRD1 Recombinant Rabbit Monoclonal Antibody (Product # 702593, 1 µg/mL). Cells were then incubated with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate for 2 h at room temperature, mounted and examined using a laser scanning confocal microscope. Note, robust receptor internalization after agonist exposure.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sections of Rat brain striatum & hypothalamus were dewaxed, microwaved in citric acid and incubated with Anti-DRD1 Recombinant Rabbit Monoclonal Antibody (Product # 702593, 1 µg/mL, 1:500 dilution). Sections were then sequentially treated with biotinylated Anti-rabbit IgG and AB solution. Sections were further developed in AEC and lightly counterstained with hematoxylin. Panel a & b) shows the specific localization of DRD1 in neuronal stomata and dendrites in striatum and hypothalamus respectively. Panel c) shows the no primary antibody control.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4. Trans -dual nicks with RecOFAR facilitates efficient gene KI in rats. ( A ) Schematic overview of the strategy to generate a GFAP-p2A-mEYFP KI allele. The nested PCR primers used for knock-in identification are shown ( Supplementary Table S4 ). Two targets are shown with green. The restriction endonuclease used for Southern blotting is SphI. A 3' internal probe on 3' homology arm is shown. ( B ) Germline transmission rate of p2A-mEYFP precise integration at the gfap locus without and with RecOFAR. ( C ) Immunostaining brain sections of gfap KI-positive F 1 rats. Neurons marked with NeuN are in red, mEYFP is in green and glial cells marked with anti-GFAP are in white. Scale bar in left panel is 200 mum. The region in the box is magnified in the right panels. Scale bar, 100 mum. ( D ) Southern blotting analysis of the gfap targeted allele and WT. T, KI target band, 6.6 kb. ( E ) Schematic overview of the strategy to generate the Drd2-p2A-ChR2-EYFP, Drd1-p2A-ChR2-EYFP and Bassoon -5.5 kb KI alleles. The restriction endonuclease HindIII and KpnI were used for Southern blotting at the Drd2 and Drd1 loci respectively. Two 3' internal probes on 3' homology arms are shown at the Drd2 and Drd1 loci. The PCR primers used for knock-in identification are shown ( Supplementary Table S4 ). ( F ) Germline transmission rate of p2A-ChR2-EYFP precise integration at the Drd2 locus with different strategies. ( G ) Southern blotting analysis of the Drd2-p2A-ChR2-EYFP targeted allele. (