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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-27172 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DRD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: IMR32.
- Concentration
- 2 mg/mL
Submitted references Activation of dopamine receptor D1 promotes osteogenic differentiation and reduces glucocorticoid-induced bone loss by upregulating the ERK1/2 signaling pathway.
Zhu J, Feng C, Zhang W, Wang Z, Zhong M, Tang W, Wang Z, Shi H, Yin Z, Shi J, Huang Y, Xiao L, Geng D, Wang Z
Molecular medicine (Cambridge, Mass.) 2022 Feb 21;28(1):23
Molecular medicine (Cambridge, Mass.) 2022 Feb 21;28(1):23
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DRD1 Polyclonal Antibody (Product # PA5-27172). Sample (30 µg of whole cell lysate). Lane A: IMR32 10% SDS PAGE. DRD1 Polyclonal Antibody (Product # PA5-27172) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of DRD1 was performed in DIV10 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: DRD1 Polyclonal Antibody (Product # PA5-27172) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of DRD1 was performed in frozen-sectioned mouse cerebral cortex tissue using DRD1 Polyclonal Antibody (Product # PA5-27172) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Dopamine Receptor D1 (Product # PA5-27172) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
