MA5-27629

antibody from Invitrogen Antibodies

Targeting: PCLO ACZ, DKFZp779G1236, KIAA0559

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27629

Provider

Invitrogen Antibodies

Product name

PCLO Monoclonal Antibody (6H9-B6)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

1 µg/mL of MA5-27629 was sufficient for detection of Piccolo in 20 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.|Detects approximately 550kDa. No cross-reactivity to Bassoon.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

6H9-B6

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PCLO in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with PCLO monoclonal antibody (Product # MA5-27629) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Piccolo Antibody, D) Merged image. Magnification: 60x.