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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MA5-27629 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCLO Monoclonal Antibody (6H9-B6)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- 1 µg/mL of MA5-27629 was sufficient for detection of Piccolo in 20 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.|Detects approximately 550kDa. No cross-reactivity to Bassoon.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6H9-B6
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCLO in rat and mouse brain lysates. The sample was incubated with PCLO monoclonal antibody (Product # MA5-27629) using a dilution of 1:1000. Samples were arranged as follows: 1) Mouse brain lysate, 2) Rat brain lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PCLO in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with PCLO monoclonal antibody (Product # MA5-27629) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Piccolo Antibody, D) Merged image. Magnification: 60x.
