LS-C793551

antibody from LSBio

Targeting: DCLK3 DCAMKL3, DCDC3C, KIAA1765

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C793551

Provider

LSBio

Product name

DCLK3 / CLR Antibody (N-Terminus) LS-C793551

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot: DCLK3 Antibody - Western Blot was performed using affinity purified SEQer DCAMKL3 antibody, aa(138-237) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml. (1:10,000 dilution). The blot was probed overnight with the SEQer DCAMKL3 antibody, aa(138-237) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence. (film exposure 5-30sec). The protein was detected as represented by the band shown.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot: DCLK3 Antibody - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight: 74 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa. (+/-20%) with additional bands present.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ELISA: DCLK3 Antibody

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum shows cytoplasmic and nuclear positivity in Purkinje cells. Antibody dilution: 1:400. Image and statement courtesy of the Human Protein Atlas. (HPA).