TA810777

antibody from OriGene

Targeting: APOBEC4 C1orf169, FLJ25691, MGC26594, RP1-127C7.4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA810777

Provider

OriGene

Product name

APOBEC4 mouse monoclonal antibody,clone OTI2A6

Antibody type

Monoclonal

Description

APOBEC4 mouse monoclonal antibody,clone OTI2A6

Host

Mouse

Conjugate

Unconjugated

Epitope

APOBEC4

Isotype

IgG

Antibody clone number

OTI2A6

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APOBEC4 (RC205405, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APOBEC4.(1:2000)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from HT29 cell line and mouse testis tissue lysate by using anti-APOBEC4 monoclonal antibody .(1:500)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-APOBEC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA810777) (1:500)

Validation comment

IHC