CF810777

antibody from Invitrogen Antibodies

Targeting: APOBEC4 C1orf169, FLJ25691, MGC26594, RP1-127C7.4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

CF810777

Provider

Invitrogen Antibodies

Product name

APOBEC4 Monoclonal Antibody (OTI2A6), TrueMAB™

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

OTI2A6

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APOBEC4 (RC205405, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-APOBEC4. (1:2000)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts (35 µg) from HT29 cell line and mouse testis tissue lysate by using anti-APOBEC4 monoclonal antibody. (1:500)

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human testicle tissue within the normal limits using anti-APOBEC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA810777)(1:500).