H00000319-M02

antibody from Abnova Corporation

Targeting: APOF

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000319-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000319-M02, RRID:AB_1111670

Product name

APOF monoclonal antibody (M02), clone 1D5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant APOF.

Antigen sequence

TSYGKQTNVLMHFPLSLESQTPSSDPLSCQFLHPK
SLPGFSHMAPLPKFLVSLALRNDALEEAGCQADVW
ALQLQLYRQGGVNATQVLIQHLRGLQKGRSTERNV
SVEALASALQLLAREQQSTGRVGRSLPTEDCENEK
EQAVHNVVQLLPGVGTFYNLGTALYYATQNCLGKA
RERGRDGAIDLGYDLLMTMAGMSGGPMGLAISAAL
KPALRSGVQQLIQYYQDQKDANISQPETTKEGLRA
ISDVSDLEETTTLASFISEVVSSAPYWGWAIIKSY
DLDPGAGSLEI


Isotype

IgG

Antibody clone number

1D5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of APOF expression in transfected 293T cell line by APOF monoclonal antibody (M02), clone 1D5.Lane 1: APOF transfected lysate(35.4 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged APOF is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to APOF on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol