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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00057085-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00057085-M02, RRID:AB_536647
- Product name
- Mouse Monoclonal AGTRAP Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. AGTRAP - angiotensin II receptor-associated protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AGTRAP Antibody (1G2) [H00057085-M02] - Analysis of AGTRAP over-expressed 293 cell line, cotransfected with AGTRAP Validated Chimera RNAi ( Cat # H00057085-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AGTRAP monoclonal antibody (M02), clone 1G2 (Cat # H00057085-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AGTRAP Antibody (1G2) [H00057085-M02] - Analysis of AGTRAP expression in transfected 293T cell line by AGTRAP monoclonal antibody (M02), clone 1G2.Lane 1: AGTRAP transfected lysate(16.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AGTRAP Antibody (1G2) [H00057085-M02] - AGTRAP monoclonal antibody (M02), clone 1G2 Analysis of AGTRAP expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: AGTRAP Antibody (1G2) [H00057085-M02] - Detection limit for recombinant GST tagged AGTRAP is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AGTRAP Antibody (1G2) [H00057085-M02] - Analysis of monoclonal antibody to AGTRAP on formalin-fixed paraffin-embedded human prostate. Antibody concentration 0.8 ug/ml.
