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- References [24]
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- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- 11566-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11566-1-AP, RRID:AB_2141975
- Product name
- MED15 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated MED15 antibody (Cat. #11566-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Role of MED15 in Enhancing EMT and Metastasis in Bladder Cancer Through YAP1 Stabilization.
The mediator subunit complex protein MED15 promotes lipid deposition and cancer progression during hypoxia.
Mediator regulates transcriptional termination through crosstalk with pre-mRNA 3' end processing factors.
PPARG-centric transcriptional re-wiring during differentiation of human trophoblast stem cells into extravillous trophoblasts.
N-terminal half of MED14 is critical for Mediator-RNA polymerase II interaction and the resulting transcription.
The mechanism of YAP/TAZ transactivation and dual targeting for cancer therapy.
Transcriptional coactivator MED15 is required for beta cell maturation.
MED15 is upregulated by HIF-2α and promotes proliferation and metastasis in clear cell renal cell carcinoma via activation of SREBP-dependent fatty acid synthesis.
MED15 prion-like domain forms a coiled-coil responsible for its amyloid conversion and propagation.
The Mediator subunit MED20 organizes the early adipogenic complex to promote development of adipose tissues and diet-induced obesity.
Mediator subunit MDT-15/MED15 and Nuclear Receptor HIZR-1/HNF4 cooperate to regulate toxic metal stress responses in Caenorhabditis elegans.
The Mediator complex subunit MED15, a promoter of tumour progression and metastatic spread in renal cell carcinoma.
The knockdown of the Mediator complex subunit MED15 restrains urothelial bladder cancer cells' malignancy.
Tyrosine-1 of RNA Polymerase II CTD Controls Global Termination of Gene Transcription in Mammals.
MED15 overexpression in prostate cancer arises during androgen deprivation therapy via PI3K/mTOR signaling.
Mediator MED23 Links Pigmentation and DNA Repair through the Transcription Factor MITF.
Differential expression of Mediator complex subunit MED15 in testicular germ cell tumors.
Clinical and molecular implications of MED15 in head and neck squamous cell carcinoma.
Human mediator subunit MED15 promotes transcriptional activation.
Mediator MED18 subunit plays a negative role in transcription via the CDK/cyclin module.
MED15, encoding a subunit of the mediator complex, is overexpressed at high frequency in castration-resistant prostate cancer.
Mediator complex recruits epigenetic regulators via its two cyclin-dependent kinase subunits to repress transcription of immune response genes.
Identification of target genes for the CDK subunits of the Mediator complex.
Modulation of PLAGL2 transactivation by positive cofactor 2 (PC2), a component of the ARC/Mediator complex.
Liu ZX, Shi YY, Zhou DS, Chen SN, Liu XQ, Huang YT, Yang ZY, Liang YT, Huang MN, Yan Y, Qiao YK, Ma Y, Liu H, Qi L, Li M, Chan SH, Zhang XD
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2026 Feb 28;40(4):e71558
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2026 Feb 28;40(4):e71558
The mediator subunit complex protein MED15 promotes lipid deposition and cancer progression during hypoxia.
Zhang B, Zhu Y, Tang Y, Liu L, Liu Y, Li Y, Yu W, Lu L
The Journal of biological chemistry 2025 Mar;301(3):108296
The Journal of biological chemistry 2025 Mar;301(3):108296
Mediator regulates transcriptional termination through crosstalk with pre-mRNA 3' end processing factors.
Lu C, Bai X, Zhang H, Zhang Y, Yang M, Zheng Y, Jin Z, Yang W, Guo G, Huang Q, Huang Y, Wu L, Fu XD, Zhang Z, Wang G
Molecular cell 2025 Jun 5;85(11):2147-2164.e10
Molecular cell 2025 Jun 5;85(11):2147-2164.e10
PPARG-centric transcriptional re-wiring during differentiation of human trophoblast stem cells into extravillous trophoblasts.
Guo Q, Choi J, Lee M, Kim J
Nucleic acids research 2025 Jul 19;53(14)
Nucleic acids research 2025 Jul 19;53(14)
N-terminal half of MED14 is critical for Mediator-RNA polymerase II interaction and the resulting transcription.
Baris Y, Jabbar J, Yozgat Y, Dinccelik-Aslan M, Cigirgan E, Erden M, Bay S, Aslan V, Cevher MA
The Journal of biological chemistry 2025 Dec;301(12):110837
The Journal of biological chemistry 2025 Dec;301(12):110837
The mechanism of YAP/TAZ transactivation and dual targeting for cancer therapy.
Yu M, Wang J, Zhang X, Zhang H, Li C, Li J, Lin J, Zheng J, Huang L, Li Y, Sun S
Nature communications 2025 Apr 24;16(1):3855
Nature communications 2025 Apr 24;16(1):3855
Transcriptional coactivator MED15 is required for beta cell maturation.
Kadhim AZ, Vanderkruk B, Mar S, Dan M, Zosel K, Xu EE, Spencer RJ, Sasaki S, Cheng X, Sproul SLJ, Speckmann T, Nian C, Cullen R, Shi R, Luciani DS, Hoffman BG, Taubert S, Lynn FC
Nature communications 2024 Oct 8;15(1):8711
Nature communications 2024 Oct 8;15(1):8711
MED15 is upregulated by HIF-2α and promotes proliferation and metastasis in clear cell renal cell carcinoma via activation of SREBP-dependent fatty acid synthesis.
Hua X, Ge S, Zhang L, Jiang Q, Chen J, Xiao H, Liang C
Cell death discovery 2024 Apr 22;10(1):188
Cell death discovery 2024 Apr 22;10(1):188
MED15 prion-like domain forms a coiled-coil responsible for its amyloid conversion and propagation.
Batlle C, Calvo I, Iglesias V, J Lynch C, Gil-Garcia M, Serrano M, Ventura S
Communications biology 2021 Mar 26;4(1):414
Communications biology 2021 Mar 26;4(1):414
The Mediator subunit MED20 organizes the early adipogenic complex to promote development of adipose tissues and diet-induced obesity.
Tang WS, Weng L, Wang X, Liu CQ, Hu GS, Yin ST, Tao Y, Hong NN, Guo H, Liu W, Wang HR, Zhao TJ
Cell reports 2021 Jul 6;36(1):109314
Cell reports 2021 Jul 6;36(1):109314
Mediator subunit MDT-15/MED15 and Nuclear Receptor HIZR-1/HNF4 cooperate to regulate toxic metal stress responses in Caenorhabditis elegans.
Shomer N, Kadhim AZ, Grants JM, Cheng X, Alhusari D, Bhanshali F, Poon AF, Lee MYY, Muhuri A, Park JI, Shih J, Lee D, Lee SV, Lynn FC, Taubert S
PLoS genetics 2019 Dec;15(12):e1008508
PLoS genetics 2019 Dec;15(12):e1008508
The Mediator complex subunit MED15, a promoter of tumour progression and metastatic spread in renal cell carcinoma.
Weiten R, Müller T, Schmidt D, Steiner S, Kristiansen G, Müller SC, Ellinger J, Syring I
Cancer biomarkers : section A of Disease markers 2018;21(4):839-847
Cancer biomarkers : section A of Disease markers 2018;21(4):839-847
The knockdown of the Mediator complex subunit MED15 restrains urothelial bladder cancer cells' malignancy.
Syring I, Weiten R, Müller T, Schmidt D, Steiner S, Kristiansen G, Müller SC, Ellinger J
Oncology letters 2018 Sep;16(3):3013-3021
Oncology letters 2018 Sep;16(3):3013-3021
Tyrosine-1 of RNA Polymerase II CTD Controls Global Termination of Gene Transcription in Mammals.
Shah N, Maqbool MA, Yahia Y, El Aabidine AZ, Esnault C, Forné I, Decker TM, Martin D, Schüller R, Krebs S, Blum H, Imhof A, Eick D, Andrau JC
Molecular cell 2018 Jan 4;69(1):48-61.e6
Molecular cell 2018 Jan 4;69(1):48-61.e6
MED15 overexpression in prostate cancer arises during androgen deprivation therapy via PI3K/mTOR signaling.
Offermann A, Vlasic I, Syring I, Vogel W, Ruiz C, Zellweger T, Rentsch CA, Hagedorn S, Behrends J, Nowak M, Merseburger A, Bubendorf L, Kirfel J, Duensing S, Adler D, Perner S
Oncotarget 2017 Jan 31;8(5):7964-7976
Oncotarget 2017 Jan 31;8(5):7964-7976
Mediator MED23 Links Pigmentation and DNA Repair through the Transcription Factor MITF.
Xia M, Chen K, Yao X, Xu Y, Yao J, Yan J, Shao Z, Wang G
Cell reports 2017 Aug 22;20(8):1794-1804
Cell reports 2017 Aug 22;20(8):1794-1804
Differential expression of Mediator complex subunit MED15 in testicular germ cell tumors.
Klümper N, Syring I, Offermann A, Adler D, Vogel W, Müller SC, Ellinger J, Strauß A, Radzun HJ, Ströbel P, Brägelmann J, Perner S, Bremmer F
Diagnostic pathology 2015 Sep 17;10:165
Diagnostic pathology 2015 Sep 17;10:165
Clinical and molecular implications of MED15 in head and neck squamous cell carcinoma.
Adler D, Offermann A, Halbach R, Vogel W, Braun M, Kristiansen G, Bootz F, Wenzel J, Mikut R, Lengerke C, Reischl M, Schröck A, Perner S
The American journal of pathology 2015 Apr;185(4):1114-22
The American journal of pathology 2015 Apr;185(4):1114-22
Human mediator subunit MED15 promotes transcriptional activation.
Nakatsubo T, Nishitani S, Kikuchi Y, Iida S, Yamada K, Tanaka A, Ohkuma Y
Drug discoveries & therapeutics 2014 Oct;8(5):212-7
Drug discoveries & therapeutics 2014 Oct;8(5):212-7
Mediator MED18 subunit plays a negative role in transcription via the CDK/cyclin module.
Kumafuji M, Umemura H, Furumoto T, Fukasawa R, Tanaka A, Ohkuma Y
Genes to cells : devoted to molecular & cellular mechanisms 2014 Jul;19(7):582-93
Genes to cells : devoted to molecular & cellular mechanisms 2014 Jul;19(7):582-93
MED15, encoding a subunit of the mediator complex, is overexpressed at high frequency in castration-resistant prostate cancer.
Adler D, Menon R, Braun M, Offermann A, Queisser A, Boehm D, Vogel W, Rüenauver K, Ruiz C, Zellweger T, Svensson M, Andren O, Kristiansen G, Wernert N, Bubendorf L, Kirfel J, Biskup S, Perner S
International journal of cancer 2014 Jul 1;135(1):19-26
International journal of cancer 2014 Jul 1;135(1):19-26
Mediator complex recruits epigenetic regulators via its two cyclin-dependent kinase subunits to repress transcription of immune response genes.
Tsutsui T, Fukasawa R, Shinmyouzu K, Nakagawa R, Tobe K, Tanaka A, Ohkuma Y
The Journal of biological chemistry 2013 Jul 19;288(29):20955-20965
The Journal of biological chemistry 2013 Jul 19;288(29):20955-20965
Identification of target genes for the CDK subunits of the Mediator complex.
Tsutsui T, Fukasawa R, Tanaka A, Hirose Y, Ohkuma Y
Genes to cells : devoted to molecular & cellular mechanisms 2011 Dec;16(12):1208-18
Genes to cells : devoted to molecular & cellular mechanisms 2011 Dec;16(12):1208-18
Modulation of PLAGL2 transactivation by positive cofactor 2 (PC2), a component of the ARC/Mediator complex.
Wezensky SJ, Hanks TS, Wilkison MJ, Ammons MC, Siemsen DW, Gauss KA
Gene 2010 Feb 15;452(1):22-34
Gene 2010 Feb 15;452(1):22-34
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP(MED15 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The MED15 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human MED15. This antibody recognizes human, mouse, rat antigen. The MED15 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
