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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A300-665A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PARP10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PARP10 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, LNCaP, GaMG, RKO, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARP10 antibody (Product # A300-665A; lot A300-665A-3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PARP10 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 10 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PARP10 antibody BL2492 (Cat. No. A300-665A) used for WB at 1 µg/ml (A and B) and used for IP at 3 µg/mg lysate. PARP10 was also immunoprecipitated using rabbit anti-PARP10 antibodies BL2491 and BL2493, which recognize upstream and downstream epitopes, respectively. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PARP10 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-PARP10 (Product # A300-665A; lot 3) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PARP10 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PARP10 (Cat. No. A300-665A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PARP10 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PARP10 antibody (Product # A300-665A; lot A300-665A-3) used for IP at 6 µg per reaction. PARP10 was also immunoprecipitated by a previous lot of this antibody (lot A300-665A-2) and rabbit anti-PARP10 antibody BL2493. For blotting immunoprecipitated PARP10 (Product # A300-665A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
