H00009693-M01

antibody from Abnova Corporation

Targeting: RAPGEF2 DKFZP586O1422, KIAA0313, PDZ-GEF1, PDZGEF1, RA-GEF

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00009693-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00009693-M01, RRID:AB_437094

Product name

RAPGEF2 monoclonal antibody (M01), clone 1E8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant RAPGEF2.

Antigen sequence

PITDFPEGHSHPARKPPDYNVALQRSRMVARSSDT
AGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLA
PYQSQGFSTEEDEDEQVSAV


Isotype

IgG

Antibody clone number

1E8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Rap2A links intestinal cell polarity to brush border formation.

Gloerich M, ten Klooster JP, Vliem MJ, Koorman T, Zwartkruis FJ, Clevers H, Bos JL
Nature cell biology 2012 Aug;14(8):793-801

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Epac1 and PDZ-GEF cooperate in Rap1 mediated endothelial junction control.

Pannekoek WJ, van Dijk JJ, Chan OY, Huveneers S, Linnemann JR, Spanjaard E, Brouwer PM, van der Meer AJ, Zwartkruis FJ, Rehmann H, de Rooij J, Bos JL
Cellular signalling 2011 Dec;23(12):2056-64

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PKC-theta selectively controls the adhesion-stimulating molecule Rap1.

Letschka T, Kollmann V, Pfeifhofer-Obermair C, Lutz-Nicoladoni C, Obermair GJ, Fresser F, Leitges M, Hermann-Kleiter N, Kaminski S, Baier G
Blood 2008 Dec 1;112(12):4617-27

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RAPGEF2 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol