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LearnA301-966A
antibody from Invitrogen Antibodies
Targeting: RAPGEF2
DKFZP586O1422, KIAA0313, PDZ-GEF1, PDZGEF1, RA-GEF
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A301-966A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDZ-GEF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PDZ-GEF1 by western blot. Samples:Whole cell lysate (50 µg) from HeLa, Hep-G2, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDZ-GEF1 antibody (Product # A301-966A; lot A301-966A-4) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse PDZ-GEF1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PDZ-GEF1 antibody A301-966A (lot A301-966A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PDZ-GEF1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PDZ-GEF1 (Product # A301-966A; lot 4) used at a dilution of 1:5,000 (0.2 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PDZ-GEF1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PDZ-GEF1 (Cat. No. A301-966A Lot3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PDZ-GEF1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PDZ-GEF1 antibody (Product # A301-966A; lot A301-966A-4) used for IP at 6 µg per reaction. For blotting immunoprecipitated PDZ-GEF1 (Product # A301-966A) was used at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
