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LearnNBP1-06549
antibody from Novus Biologicals
Targeting: RAPGEF2
DKFZP586O1422, KIAA0313, PDZ-GEF1, PDZGEF1, RA-GEF
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NBP1-06549 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-06549, RRID:AB_1582456
- Product name
- Rabbit Polyclonal RAPGEF2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Protein Kinase A-independent Ras Protein Activation Cooperates with Rap1 Protein to Mediate Activation of the Extracellular Signal-regulated Kinases (ERK) by cAMP.
NEDD4-1 regulates migration and invasion of glioma cells through CNrasGEF ubiquitination in vitro.
Rapgef2 connects GPCR-mediated cAMP signals to ERK activation in neuronal and endocrine cells.
Li Y, Dillon TJ, Takahashi M, Earley KT, Stork PJ
The Journal of biological chemistry 2016 Oct 7;291(41):21584-21595
The Journal of biological chemistry 2016 Oct 7;291(41):21584-21595
NEDD4-1 regulates migration and invasion of glioma cells through CNrasGEF ubiquitination in vitro.
Zhang H, Nie W, Zhang X, Zhang G, Li Z, Wu H, Shi Q, Chen Y, Ding Z, Zhou X, Yu R
PloS one 2013;8(12):e82789
PloS one 2013;8(12):e82789
Rapgef2 connects GPCR-mediated cAMP signals to ERK activation in neuronal and endocrine cells.
Emery AC, Eiden MV, Mustafa T, Eiden LE
Science signaling 2013 Jun 25;6(281):ra51
Science signaling 2013 Jun 25;6(281):ra51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAPGEF2 Antibody [NBP1-06549] - Detection of Human and Mouse PDZ-GEF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PDZ-GEF1 antibody NBP1-06549 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAPGEF2 Antibody [NBP1-06549] - Whole cell lysate (50 ug) from HeLa, Hep-G2, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDZ-GEF1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RAPGEF2 Antibody [NBP1-06549] - Detection of human PDZ-GEF1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PDZ-GEF1 antibody NBP1-06549 used for IP at 6 ug per reaction. For blotting immunoprecipitated PDZ-GEF1, NBP1-06549 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: RAPGEF2 Antibody [NBP1-06549] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PDZ-GEF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RAPGEF2 Antibody [NBP1-06549] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PDZ-GEF1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
