AP6650d

antibody from Abcepta

Targeting: GLS2 GA, GLS, hLGA, LGA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP6650d

Provider

Abcepta

Proper citation

Abgent Cat#AP6650d, RRID:AB_10818414

Product name

GLS2 Antibody (C-term E513)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Western blot analysis of lysate from human brain tissue lysate, using GLS2 Antibody (C-term E513)(Cat. #AP6650d). AP6650d was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug."

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

GLS2 Antibody (C-term E513) (Cat. #AP6650d) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the GLS2 antibody detected the GLS2 protein (arrow).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

GLS2 antibody(C-term E513) (Cat. #AP6650d) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLS2 antibody(C-term E513) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50