PA5-78475

antibody from Invitrogen Antibodies

Targeting: GLS2 GA, GLS, hLGA, LGA

Antibody data

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Validation data

Product number: PA5-78475 - Provider product page
Provider: Invitrogen Antibodies
Product name: GLS2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Positive Control: Huh-7, HepG2, mouse liver, rat liver Predicted Reactivity: Bovine (100%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.17 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

GLS2 protein structure - PA5-78475 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GLS2 in rat tissue extract using 50 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with GLS2 polyclonal antibody (Product # PA5-78475) using a dilution of 1:1000 followed by HRP-conjugated anti-rabbit IgG.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GLS2 in mouse tissue extract using 50 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with GLS2 polyclonal antibody (Product # PA5-78475) using a dilution of 1:1000 followed by HRP-conjugated anti-rabbit IgG.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GLS2 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with GLS2 polyclonal antibody (Product # PA5-78475) using a dilution of 1:1000 followed by HRP-conjugated anti-rabbit IgG.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using GLS2 Polyclonal Antibody (Product # PA5-78475). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GLS2 Polyclonal Antibody (Product # PA5-78475) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using GLS2 Polyclonal Antibody (Product # PA5-78475). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GLS2 Polyclonal Antibody (Product # PA5-78475) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 Change of protein levels of PER3, GLS2, CREM and TXNIP in AT1 prostate and Walker-256 mammary carcinoma cells after 24 h under acidotic (pH 6.6) and/or hypoxic (pO 2 = 1.5 mmHg) conditions. n = 4-12; (*) p < 0.05, (**) p < 0.01 vs. control; ( # ) p < 0.05, ( ## ) p < 0.01 AT1 vs. Walker-256 cells

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 Protein levels of PER3, GLS2, CREM and TXNIP in AT1 prostate and Walker-256 mammary carcinoma tumors in vivo 24 h after ( a ) inducing metabolic acidosis by forcing glycolytic metabolism or ( b ) intratumoral injection of lactic acid. n = 4-18; (*) p < 0.05, (**) p < 0.01 vs. control; ( # ) p < 0.05, ( ## ) p < 0.01 AT1 vs. Walker-256 cells

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Cisplatin-resistant CRC cells exhibit elevated glutamine metabolism (A) Heatmap of differentially expressed genes from LoVo parental and CDDP R cells identified by RNA-seq. (B) MetaboAnalyst pathway enrichment analysis of metabolites in LoVo parental and CDDP R cells. (C) mRNA expression levels of glutamine metabolism enzymes and regulators were detected by qRT-PCR in LoVo parental and CDDP R cells. (D) Western blot results show protein expression of GLS1 and GLS2 in LoVo parental and CDDP R cells. (E and F) Glutamine uptake (E) and GLS activity (F) assays were performed in LoVo parental and CDDP R cells. (G) LoVo parental and CDDP R cells were cultured with regular medium or glutamine depletion medium, a clonogenic assay and (H) annexin V assay were performed. (I) LoVo CDDP R cells cultured with regular medium or glutamine depletion medium were treated with cisplatin at 10 and 20 muM for 48 h. (J) Cell viability and cell death were examined by a clonogenic assay and (H) annexin V assay. Columns include mean of three independent experiments; data are presented as mean +- SD. *p < 0.05, **p < 0.01, ***p < 0.001.