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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ABIN950280 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Adenylate Cyclase 7 (Adcy7) (AA 517-547), (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 517~547 amino acids from the Center region of human ADCY7
- Description
- Affinity chromatography on Protein A
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 517-547,Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of ADCY7 Antibody (Center) (Cat. #AP50088PU-N) in MCF-7 cell line lysates (35 μg/lane). ADCY7 (arrow) was detected using the purified Pab
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma reacted with ADCY7 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ADCY7 Antibody (Center) (Cat. #AP50088PU-N) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
