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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MAB7456 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Endophilin B1/B2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Endophilin B1/Bif-1 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human Endophilin B2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y371- Isotype
- IgG
- Antibody clone number
- 807009
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Endophilin B1/B2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Saos-2 human osteosarcoma cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Endophilin B1/B2 Monoclonal Antibody (Catalog # MAB7456) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Endophilin B1/B2 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Endophilin B1/B2 by Simple WesternTM. Simple Western lane view shows lysates of U-118-MG human glioblastoma/astrocytoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Endophilin B1/B2 at approximately 42 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human Endophilin B1/B2 Monoclonal Antibody (Catalog # MAB7456). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Endophilin B1/B2 in Jurkat Human Cell Line. Endophilin B1/B2 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human Endophilin B1/B2 Monoclonal Antibody (Catalog # MAB7456) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Endophilin B1/B2 in Human Pancreas. Endophilin B1/B2 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Endophilin B1/B2 Monoclonal Antibody (Catalog # MAB7456) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
