Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-53340 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Bif1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DRKAPSRINN PELLGQYMID AGTEFGPGTA YGNALIKCGE TQKRIGTADR ELIQTSALNF LTPLRNFIEG DYKTIAKER Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Bif1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a Bif1 Polyclonal Antibody (Product # PA5-53340).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Bif1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a Bif1 Polyclonal Antibody (Product # PA5-53340).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Bif1 in human cell line U-251 MG shows positivity in cytoplasm. Samples were probed using a Bif1 Polyclonal Antibody (Product # PA5-53340).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Bif1 in human cell line U-251 MG using Bif1 Polyclonal Antibody (Product # PA5-53340) shows localization to cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Bif1 in human testis using Bif1 Polyclonal Antibody (Product # PA5-53340) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Bif1 in human pancreas using Bif1 Polyclonal Antibody (Product # PA5-53340) shows low expression as expected.