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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NBP1-31130 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-31130, RRID:AB_2301707
- Product name
- Rabbit Polyclonal Endophilin B1/Bif-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Inhibition of autophagic turnover in β-cells by fatty acids and glucose leads to apoptotic cell death.
Mir SU, George NM, Zahoor L, Harms R, Guinn Z, Sarvetnick NE
The Journal of biological chemistry 2015 Mar 6;290(10):6071-85
The Journal of biological chemistry 2015 Mar 6;290(10):6071-85
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Endophilin B1/Bif-1 Antibody [NBP1-31130] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Endophilin B1/Bif-1 Antibody [NBP1-31130] - (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE, antibody diluted at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NBP1-31130] - Paraffin-embedded U87 xenograft; antibody dilution 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NBP1-31130] - Bif-1 antibody detects Bif-1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Bif-1 antibody (NBP1-31130). Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
