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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-28068 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Bif1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: H1299, NIH-3T3, JC, BCL-1.
- Concentration
- 0.57 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Bif1 Polyclonal Antibody (Product # PA5-28068). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC. Lane C: BCL-1. 10% SDS PAGE. Bif1 Polyclonal Antibody (Product # PA5-28068) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Bif1 Polyclonal Antibody (Product # PA5-28068). Sample (30 µg of whole cell lysate). A: H1299. 7.5% SDS PAGE. Bif1 Polyclonal Antibody (Product # PA5-28068) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Bif1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 10 min. Green: Bif1 Polyclonal Antibody (Product # PA5-28068) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Bif-1 antibody detects Bif-1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Bif-1 antibody (Product # PA5-28068) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
