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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- AP7171a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP7171a, RRID:AB_2163930
- Product name
- PIM3 Antibody (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references PIM kinases are progression markers and emerging therapeutic targets in diffuse large B-cell lymphoma.
Pim kinase inhibitor, SGI-1776, induces apoptosis in chronic lymphocytic leukemia cells.
KSHV reactivation from latency requires Pim-1 and Pim-3 kinases to inactivate the latency-associated nuclear antigen LANA.
Brault L, Menter T, Obermann EC, Knapp S, Thommen S, Schwaller J, Tzankov A
British journal of cancer 2012 Jul 24;107(3):491-500
British journal of cancer 2012 Jul 24;107(3):491-500
Pim kinase inhibitor, SGI-1776, induces apoptosis in chronic lymphocytic leukemia cells.
Chen LS, Redkar S, Bearss D, Wierda WG, Gandhi V
Blood 2009 Nov 5;114(19):4150-7
Blood 2009 Nov 5;114(19):4150-7
KSHV reactivation from latency requires Pim-1 and Pim-3 kinases to inactivate the latency-associated nuclear antigen LANA.
Cheng F, Weidner-Glunde M, Varjosalo M, Rainio EM, Lehtonen A, Schulz TF, Koskinen PJ, Taipale J, Ojala PM
PLoS pathogens 2009 Mar;5(3):e1000324
PLoS pathogens 2009 Mar;5(3):e1000324
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- The anti-PIM3 Pab (Cat. #AP7171a) is used in Western blot to detect PIM3 in mouse kidney tissue lysate.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PIM3 antibody (C-term)(Cat.#AP7171a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:10~50
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human Spleen tissue reacted with PIM3 antibody (C-term)(Cat.#AP7171a), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:10~50
