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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00055319-M08 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00055319-M08, RRID:AB_10549801
- Product name
- C4orf43 monoclonal antibody (M08), clone 4A6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant C4orf43.
- Antigen sequence
MPKAPKGKSAGREKKVIHPYSRKAAQITREAHKQE
KKEKLKNEKALRLNLVGEKLQWFQNHLDPPKKRYS
KKDACELIERYLNRFSSELEQIELHNSIRDRQGRR
HCSRETVIKQTMERERQQFEGYGLEIPDILNASNL
KTFREWDFDLKKLPNIKMRKICANDAIPKTCKRKT
TITVDQDLGELELNDESSDSDEEMTAVA- Isotype
- IgG
- Antibody clone number
- 4A6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of C4orf43 expression in transfected 293T cell line by C4orf43 monoclonal antibody (M08), clone 4A6.Lane 1: C4orf43 transfected lysate(23.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged C4orf43 is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to C4orf43 on formalin-fixed paraffin-embedded human breast. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
