PA5-95207

antibody from Invitrogen Antibodies

Targeting: GRIA1 GluA1, GLUR1, GLURA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-95207

Provider

Invitrogen Antibodies

Product name

GluR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Human GRIA1 shares 98% amino acid (aa) sequence identity with both mouse and rat GRIA1. Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Role of Satb1 and Satb2 Transcription Factors in the Glutamate Receptors Expression and Ca(2+) Signaling in the Cortical Neurons In Vitro.

Turovsky EA, Turovskaya MV, Fedotova EI, Babaev AA, Tarabykin VS, Varlamova EG
International journal of molecular sciences 2021 May 31;22(11)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GluR1 in Lane 1: recombinant human GRIA1 protein (0.5 ng). Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with GluR1 polyclonal antibody (Product # PA5-95207) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GluR1 in Lane 1: rat brain tissue lysate, Lane 2: mouse brain tissue lysate. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with GluR1 polyclonal antibody (Product # PA5-95207) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GluR1 in paraffin-embedded mouse brain tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with GluR1 polyclonal antibody (Product # PA5-95207) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GluR1 in paraffin-embedded rat brain tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with GluR1 polyclonal antibody (Product # PA5-95207) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GluR1 in paraffin-embedded human meningioma tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with GluR1 polyclonal antibody (Product # PA5-95207) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Expression of subunits forming AMPAR (GluA1, GluA2) and NMDAR (GluN2A, GluN2B) in neurons of the cerebral cortex derived from Satb-deficient, Satb-, and control mice. ( A , B )--Immunostaining of GluA1 (green color), GluA2 (red color) subunits of AMPA receptors ( A ) and GluN2A (green color), GluN2B (red color) of NMDA receptors ( B ) of cortical neurons from control (Satb +/+ * Nex Cre/+ ), Satb-deficient (Satb fl/+ * Nex Cre/+ ) and Satb- (Satb2 fl/fl * Nex Cre/+ ) mice. ( C - F) --The effects of Satb- and Satb2-deletions on the level of GluA1 ( C ), GluA2 ( D ), GluN2A ( E ), and GluN2B ( F ) subunits. Intensity levels of surface-expressed receptor subunits were determined by confocal imaging. We analyzed individual neurons which had fluorescence of Alexa Fluor 633 (GluA1, green color), Alexa Fluor 488 (GluA2, red color) and Alexa Fluor-555 (GluN2B, red color), and Alexa Fluor-594 (GluN2A, green color). The quantitative data reflecting the level of subunits expression are presented as fluorescence intensity values in summary bar charts (mean +- SEM). The values were averaged by 300 +- 50 neurons for each column. Statistical significance was assessed using paired t-test. The results obtained after immunostaining well agree with the data of fluorescent Ca 2+ measurements presented in Figure 4 and Figure 5 . After Ca 2+ measurements, the cells were fixed and stained by the antibodies. We used the scans from three independent view fields for each experimental