AGC-004-GP-50UL

antibody from Invitrogen Antibodies

Targeting: GRIA1 GluA1, GLUR1, GLURA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AGC-004-GP-50UL

Provider

Invitrogen Antibodies

Product name

GluR1 (GluA1) (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Guinea Pig

Isotype

IgG

Vial size

50 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysisof rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody (#AGC-004-GP), (1:200).3,4. Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody , preincubated with GluR1/GluA1 (extracellular) Blocking Peptide (#BLP-GC004).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysisof rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody (#AGC-004-GP), (1:200).3,4. Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody , preincubated with GluR1/GluA1 (extracellular) Blocking Peptide (#BLP-GC004).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Multiplex staining of GluR1 and Vesicular GABA Transporter in human U-87 MG cells - Cell surface detection of GluR1 and Vesicular GABA Transporter in human glioblastoma U-87 MG. Extracellular staining of live intact cells with Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody (#AGC-004-GP), (1:25), followed by goat Anti-guinea pig-AlexaFluor-488 secondary Antibody (green). Cells were subsequently fixed, permeabilized and labeled with Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005), (1:200), followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). Representative merged images of the double labeled cells are shown in A and B.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Multiplex staining of GluR1 and Vesicular GABA Transporter in human U-87 MG cells - Cell surface detection of GluR1 and Vesicular GABA Transporter in human glioblastoma U-87 MG. Extracellular staining of live intact cells with Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody (#AGC-004-GP), (1:25), followed by goat Anti-guinea pig-AlexaFluor-488 secondary Antibody (green). Cells were subsequently fixed, permeabilized and labeled with Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005), (1:200), followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). Representative merged images of the double labeled cells are shown in A and B.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of GluR1 in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody (#AGC-004-GP), (1:400), followed by goat Anti-guinea pig-Cy2 Antibody (green). GluR1 staining appears in neuronal outlines (horizontal arrows) and in the inner molecular layer of the dentate gyrus (vertical arrow). Nuclei are stained with DAPI (blue).