TA501547

antibody from OriGene

Targeting: KLHL2 MAV

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501547

Provider

OriGene

Proper citation

OriGene Cat#TA501547, RRID:AB_11124210

Product name

Anti-KLHL2 mouse monoclonal antibody, clone OTI8B8 (formerly 8B8)

Antibody type

Monoclonal

Description

Anti-KLHL2 mouse monoclonal antibody, clone OTI8B8 (formerly 8B8)

Host

Mouse

Conjugate

Unconjugated

Epitope

KLHL2

Isotype

IgG

Antibody clone number

OTI8B8

Vial size

100 µl

Concentration

0.74 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL2 (RC205347, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLHL2.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-KLHL2 mouse monoclonal antibody (TA501547) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KLHL2(RC205347).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC205347 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-KLHL2 antibody(TA501547), and then analyzed by flow cytometry.

Validation comment

FC