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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- CF501547 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KLHL2 Monoclonal Antibody (OTI8B8), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI8B8
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL2 (RC205347, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-KLHL2. Positive lysates LY402119 (100 µg) and LC402119 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL2 (RC205347, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-KLHL2. Positive lysates LY402119 (100 µg) and LC402119 (20 µg) can be purchased separately from OriGene.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-KLHL2 mouse monoclonal antibody (TA501547) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KLHL2(RC205347).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-KLHL2 mouse monoclonal antibody (TA501547) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KLHL2(RC205347).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC205347 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KLHL2 antibody (TA501547), and then analyzed by flow cytometry.
