- HPA061111 - Atlas Antibodies SUGP2 antibody

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Enhanced validation

Submitted by: 55af80e3e0991
Enhanced method: Genetic validation
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Experimental details: Confocal images of immunofluorescently stained human U-2 OS cells.The protein SUGP2 is shown in green and the microtubules in red. The image to the left show cells transfected with control siRNA and the image to the right show cells where SUGP2 has been downregulated with specific siRNA.
Sample type: U-2 OS cells
Primary Ab dilution: 1:109
Secondary Ab: Secondary Ab
Secondary Ab dilution: 1:800
Knockdown/Genetic Approaches Application: Immunocytochemistry
KD Reagent Type: siRNA
Downregulation: >75%
KD Reagent Provider: Thermo Fisher Scientific
KD Reagent Prod no: s19759
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Page 2: KD Reagent Page 2
KD Reagent Prod no 2: s19757
KD Reagent Page 3: KD Reagent Page 3
KD Reagent Prod no 3: s19758
Antibody Lot Number: R85544
Appendix: 55b0a4e0c1225.pdf

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Sample type: HUMAN

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human testis and pancreas tissues using Anti-SUGP2 antibody. Corresponding SUGP2 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
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Experimental details: Immunohistochemical staining of human cerebral cortex, kidney, lymph node and testis using Anti-SUGP2 antibody HPA061111 (A) shows similar protein distribution across tissues to independent antibody HPA041557 (B).
Antibody #2 product nr: HPA041557
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human testis shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human pancreas shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney using Anti-SUGP2 antibody HPA061111.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human lymph node using Anti-SUGP2 antibody HPA061111.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex using Anti-SUGP2 antibody HPA061111.
Sample type: HUMAN

HPA061111