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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-35979 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SUGP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: HeLa, HeLa NE, Neuro 2A, C2C12. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SUGP2 Polyclonal Antibody detects SFRS14 protein by western blot analysis. A. 30 µg HeLa whole cell extract. B. 30 µg HeLa nuclear extract.5 % SDS-PAGE. SUGP2 Polyclonal Antibody (Product # PA5-35979) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SUGP2 Polyclonal Antibody detects SFRS14 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SUGP2 Polyclonal Antibody (Product # PA5-35979) diluted by 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of SFRS14 was performed in HeLa whole cell extracts using 5 µg of SUGP2 Polyclonal Antibody (Product # PA5-35979). Samples were transferred to a membrane and probed with SUGP2 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of SFRS14 was performed in HeLa whole cell extracts using 5 µg of SUGP2 Polyclonal Antibody (Product # PA5-35979). Samples were transferred to a membrane and probed with SUGP2 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
