Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A302-087A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LARP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human LARP1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LARP1 antibody (Product # A302-087A; lot A302-087A-3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human LARP1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LARP1 antibody (Product # A302-087A; lot A302-087A-3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human LARP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LARP1 antibody A302-087A (lot A302-087A-2) used for IP at 6 µg per reaction. LARP1 was also immunoprecipitated by rabbit anti-LARP1 antibody A302-088A. For blotting immunoprecipitated LARP1, A302-087A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human LARP1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LARP1 antibody A302-087A (lot A302-087A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human LARP1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-LARP1 (Product # A302-087A; lot 3) used at a dilution of 1:200 (1 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human LARP1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-LARP1 (Cat. No. A302-087A Lot2) used at a dilution of 1:200 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human LARP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LARP1 antibody (Product # A302-087A; lot A302-087A-3) used for IP at 3 µg per reaction. LARP1 was also immunoprecipitated by rabbit anti-LARP1 antibody (Product # A302-088A). For blotting immunoprecipitated LARP1 (Product # A302-087A) was used at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
